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Proximate Composition, Chlorophyll a, and Carotenoid Content in Dunaliella salina (Dunal) Teod (Chlorophycea: Dunaliellaceae) Cultured with Cost-Effective Seaweed Liquid Fertilizer Medium
Seaweed liquid fertilizer Dunaliella salina Algal culture
2010/3/17
Growth, proximate composition, chlorophyll a, and carotenoid content were estimated in static cultures of micro alga Dunaliella salina (Dunal) Teod (Chlorophycea: Dunaliellaceae) to compare the qualit...
盐藻hsp70a cDNA 片段的克隆与分析Cloning and Characterization of Hsp70a cDNA Fragment of Dunaliella salina
杜氏盐藻 hsp70a cDNA 简并引物
2007/12/31
摘要根据衣藻、矮牵牛花、Pisumsativum等真核生物hsp70a氨基酸高度保守序列设计两对简并引物，以热休克盐藻cDNA为模板进行巢式PCR扩增，产物经T-A克隆转化至JM109大肠杆菌中，经筛选后测序，将测序结果推导成氨基酸序列进行同源性分析，获得了盐藻热休克蛋白70a cDNA片段。在盐藻中所获得的cDNA片段，核苷酸长度为372 bp，编码126个氨基酸。推导的氨基酸序列与与下列物种的...