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Rapid Isolation of Bacterial Photosynthetic Reaction Centers with an Engineered Poly-Histidine Tag
photosynthetic reaction center poly-histidine tag isolation method (bacterium)
2016/5/23
A very rapid method for isolating bacterial photosynthetic reaction centers (RCs) of high purity and yield is described. A poly-histidine tag is engineered at the C-terminus of the RC M-subunit, allow...
Isolation and Characterization of a New Methanobacterium formicicum KOR-1 from an Anaerobic Digester Using Pig Slurry
Methanogen Anaerobic Digester 16S rRNA mcrA Methanobacterium formicicum
2016/5/20
A new methanogen was isolated from an anaerobic digester using pig slurry in South Korea. Only one strain, designated KOR-1, was characterized in detail. Cells of KOR-1 were straight or crooked rods, ...
Lactic Acid Bacteria in Total Mixed Ration Silage Containing Soybean Curd Residue: Their Isolation, Identification and Ability to Inhibit Aerobic Deterioration
Soybean Curd Residue–Containing TMR Silage Aerobic Deterioration Lactic Acid Bacteria Synergistic Effect
2016/5/20
We investigated the effects of the predominant lactic acid bacteria (LAB) on the fermentation characteristics and aerobic stability of total mixed ration (TMR) silage containing soybean curd residue (...
Urinary Catecholamine and Cortisol Responses of Japanese Shorthorn Cows to Social Isolation
Urinary Cortisol Urinary Catecholamines Social Isolation
2016/5/3
This study was performed to investigate the use of urinary catecholamines to monitor changes in the activity of the sympathetic nervous system and to determine the relationship of urinary cortisol and...
Isolation and In vitro Culture of Pig Spermatogonial Stem Cell
Spermatogonial Stem Cell SSC Pluripotency Long Term Culture Pig
2016/4/29
The present study identified the favorable conditions for isolation, enrichment and in vitro culture of highly purified, undifferentiated pig spermatogonial stem cell (SSC) lines that proliferate for ...
Strong Reproductive Isolation between Closely Related Tropical Sea Urchins (genus Echinometra)’
Reproductive Isolation Closely Related
2015/12/17
Morphological, mitochondrial DNA, and single-copy nuclear DNA differences show
that the tropical sea urchin Echinometra mathaei is composed of at least four
independent gene pools. Evolutionary di...
GENETIC DIVERGENCE, REPRODUCTIVE ISOLATION, AND MARINE SPECIATION
allopatric speciation dispersal molecular evolutio
2015/12/17
In marine species, high dispersal is often associated with only mild genetic
differentiation over large spatial scales. Despite this generalization, there are
numerous reasons for the accumulation o...
USING ISOLATION BY DISTANCE AND EFFECTIVE DENSITY TO ESTIMATE DISPERSAL SCALES IN ANEMONEFISH
Amphiprion clarkii continuous population
2015/12/11
Robust estimates of dispersal are critical for understanding population dynamics and local adaptation, as well as for successful
spatial management. Genetic isolation by distance patterns hold clues ...
Analysis of seismic noise to check the mechanical isolation of a medical device
Seismic noise noise acquisition anthropogenic sources Spectral Ratio Analyses Magnetoencephalography
2015/8/27
We have investigated the mechanical response of a magnetically shielded room that hosts a magnetoencephalography system that is subject to external vibrations. This is a superconducting quantum interf...
High Throughput Cultivation for Isolation of Novel Marine Microorganisms
High Throughput Cultivation Novel Marine Microorganisms
2015/7/23
Natural products are organic molecules derived from plants, animals, or microorganisms, and represent the starting point for most of the anti-infective and anti-cancer drugs on the market today. Until...
Genetic Evidence for Regional Isolation of Pocillopora Corals from Moorea
Pocillopora Corals from Moorea Regional Isolation
2015/7/16
Pocillopora is one of the most abundant and widely distributed coral genera, with a geographic range spanning the entire tropical Pacific and Indian Oceans as well as the Red Sea and Arabian Gulf. Acr...
Isolation, Culture and Identification of Porcine Skeletal Muscle Satellite Cells
Pig Skeletal Muscle Satellite Cell Isolation Culture Characterization
2016/5/18
The objective of this study was to establish the optimum protocol for the isolation and culture of porcine muscle satellite cells. Mononuclear muscle satellite cells are a kind of adult stem cell, whi...
Isolation and characterization of multicytochrome gene cluster from Shewanella putrefaciens MR-1 involved in iron and manganese reduction
Biological sciences Multicytochrome gene cluster Manganese reduction Iron reduction Schewanella putrefaciens
2015/1/28
Dissimilatory Fe(III) and Mn(IV) reduction is an anaerobic respiratory process common to many environments. A large number of bacteria that utilize these metals as terminal electron acceptors have bee...
Towards the isolation of esterase and amidase catalytic antibodies: Examination of germinal center diversity by cloning and sequence analysis of genes encoding monoclonal antibodies elicited by a transition state analogue
Esterase catalytic antibodies amidase amide ester hydrolysis reaction hybrid cells B cells the TSA induced antibodies genes cells cell lines
2014/12/11
In this study, a transition state analogue (TSA) approach was employed for the isolation of catalytic antibodies with esterase or amidase activity. The TSA used in this study, phosphonamidate, mimics ...
Influence of salts on selective coagulation of whey proteins and their application in the isolation of β-lactoglobulin
whey protein concentrate electrodialysis precipitation of caseinomacropeptide separation of proteins salting out
2014/3/28
Whey proteins are an important constituent of milk, especially whey from cheese manufacture and have many valuable functional properties such as foaming and emulsifying ability or gel formation. Some ...