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Impact of cadmium on the level of hepatic metallothioneins, essential elements, and selected enzymes in the experimental rat model
metal intoxication liver blood plasma detoxification Rattus norvegicus L.
2015/7/2
The response of different strains of laboratory rats (Rattus norvegicus L.) on both acute (via intraperitoneal injection) and chronic (via drinking water and/or diet) cadmium intoxication was investig...
Delayed Anesthetic Preconditioning and Metallothioneins I+II: Novel Mediators of Anesthetic-Induced Protection
Ischemia Metallothioneins Microarray Preconditioning Protection Anesthetic preconditioning
2014/12/10
Ischemic injury is a common and debilitating outcome of natural illness and as a complication of commonly performed medical procedures. Whereas naturally occurring ischemic insults are often the resul...
小鼠肝脏金属硫蛋白Metallothioneins的分离纯化与鉴定
金属硫蛋白 小鼠肝脏金属硫蛋白 金属硫蛋白的分离
2007/12/24
摘要 由健康小鼠通过皮下注射一定量的氯化镉,取肝脏匀浆,离心,经SephadexG-50和DEAE-Sephadex A-50柱层析分离,即可获得MT-Ⅰ和MT-Ⅱ两种金属硫蛋白。经鉴定:两个分子各含18个巯基,结合4个Cd原子和3个Zn原子,无金属蛋白质Thionein的分子量约为6kD,半胱氨酸含量约占氨基酸总量的28％,所提取到的MT-Ⅰ和MT-Ⅱ经氨基酸组成,HPLC和PAGE分析皆证明为...