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Purification of Escherichia coli-expressed HIS-tagged Maedi-Visna p25 core antigen by Ni2+-chelate affinity chromatography
recombinant antigen ELISA immunoblo
2015/1/20
In this study, recombinant histidine tagged p25 capsid protein of Maedi-Visna virus was developed. Part of the viral genome coding p25 protein was positioned downstream and in frame with a metal bindi...
Purification of L-Lysine in Simulated Moving Bed and Fixed-Bed Chromatography
ion exchange chromatography simulated moving bed purification L-lysine
2009/11/18
L-Lysine was produced by a microbial process utilizing a Corynebacterium glutamicum (ATCC 21799) strain. L-Lysine was purified from the cultivated medium by fixed-bed and simulated moving bed （SMB） c...
Modeling of TaqI Endonuclease Purification by HPLC
TaqI endonuclease modeling design equilibrium theory equilibrium-dispersive theory plate theory
2010/4/22
The present work is based on the previously reported purification study of TaqI endonuclease5 and aimed to simulate the experimentally obtained chromatograms in order to reduce the time and cost in th...