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黄鳝激素敏感性脂肪酶基因Hsl染色体原位杂交定位Chromosomal Localization of the Hormone-sensitive Lipase Gene (Hsl) in Rice Field Eel
黄鳝 Hsl基因 Southern杂交 FISH
2007/12/30
摘要动物脂肪组织中的甘油三酯在数量上是最重要的储存能源。Hsl基因所编码的激素敏感性脂肪酶是一种多功能酶。它通过催化水解储存在脂肪组织中的甘油三酯，以及卵巢、肾上腺、睾丸和胎盘中的胆固醇酯，在机体能量供应和类固醇生成作用中发挥重要作用。本研究以放射性同位素和地高辛标记重组质粒pBS中所含猪Hsl基因作为探针，分别与Pst Ⅰ酶切的黄鳝基因组总DNA和有丝分裂染色体标本进行Southern杂交和荧光...
Chromosomal Localization of Korean Cattle (Hanwoo) BAC Clones via BAC end Sequence Analysis
Bovine BAC Library Pooling System BAC End Sequence Comparative Mapping Chromosomal Localization
2016/4/25
In this study, a Korean native cattle strain (Hanwoo) evidencing high performance in terms of both meat quality and quantity was employed in the generation of 150,000 BAC clones with an average insert...
Sequence Characterization, Expression Profile, Chromosomal Localization and Polymorphism of the Porcine SMPX Gene
Sequence Analysis Physical Mapping Polymorphism Promoter Porcine SMPX
2016/4/21
The full-length cDNA of the porcine SMPX gene was obtained by the rapid amplification of cDNA ends (RACE). The nucleotide sequences and the predicted protein sequences share high sequence identity wit...