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Optimization of Procedure for Efficient Gene Transfer into Porcine Somatic Cells with Lipofection
Lipofection Porcine Developmental Competence Somatic Cell Nuclear Transfer (SCNT)
2016/4/27
The objective of this study was to establish conditions for transfection of a foreign gene into somatic cells using cationic lipid reagents and to evaluate the effects of transfection on in vitro deve...
精子介导转基因动物的制备Research on Spermatozoa-mediated Gene Transfer to Product Transgenic Animals
精子 脂质体 转基因动物
2007/12/30
摘要采用直接注射的方法，将脂质体包裹的含人乳铁蛋白基因的重组质粒pLNCXHLF注入兔睾丸组织和羊输精管中，一个月后分别与正常雌兔及正常的母羊交配。所产仔兔均为活体，经PCR和Southern检测,转基因仔兔阳性率平均为35%(11/31)；羔羊经引物F1/R1系统PCR检测阳性率为33.3％（4/12），F2/R2系统PCR检测阳性率为25%（3/12）。将流产羔羊组织解剖后提取舌、肺、肝脏、肌...
Application of Gene Transfer Technology for Genetic Improvement of Fish
Transgenic fish genetic improvement of fish gene transfer aquaculture
2010/3/29
The worldwide decline of natural fish stocks, the accumulation of chemical pollutants in aquatic environments and increasing world consumption of fish products has provided an impetus in many countrie...
Follow-up of Exogenous DNA by Sperm-mediated Gene Transfer via Liposome
Gene Transfer Sperm Vector Intracytoplasmic Sperm Injection Mouse Bacterial Lacz
2016/4/12
To examine the feasibility of using a sperm vector system for gene transfer, we have investigated the binding and the uptaking of foreign DNA into the sperm nucleus by PCR, in situ hybridization and L...