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Effect of Antioxidant Flavonoids (Quercetin and Taxifolin) on In vitro Maturation of Porcine Oocytes
Porcine Oocyte Antioxidants Quercetin Reactive Oxygen Species [ROS]
2016/5/19
Quercetin (QT) and taxifolin (TF) are structurally similar plant-derived flavonoids that have antioxidant properties and act as free radical scavengers. The objective of this study was to investigate ...
Effects of oocyte collection techniques and maturation media on in vitro maturation and subsequent embryo development in Boer goat
Boer goat collection techniques oocyte maturation ovary medium
2015/6/4
The oocytes (experiment 1) were harvested by one of the four collection techniques (slicing, puncture, aspiration I and aspiration II) and the total number and the number of each grade of oocytes were...
CHARACTERIZATION OF Ca2+ INFLUX PATHWAY(S) DURING MOUSE OOCYTE MATURATION
Calcium influx oocyte maturation SOCE Stim1 Phosphorylation
2014/12/10
Ca2+ signaling induced at fertilization, also known as oscillations, is essential in mammalian eggs to initiate early embryonic development. The generation of the oscillations relies on optimization o...
Effects of Trichostatin A on Cumulus Expansion during Mouse Oocyte Maturation
Mouse Cumulus-oocyte Complex Cumulus Expansion TSA
2016/5/12
This study was conducted to investigate the effects of Trichostatin A (TSA) on cumulus expansion during mouse oocyte maturation. TSA treatment inhibited cumulus expansion and significantly reduced the...
Effect of MEM Vitamins Supplementation of In vitro Maturation Medium and In vitro Culture Medium on the Development of Porcine Embryos
Porcine Embryo Development Blastocyst Vitamins
2016/5/9
This study was carried out to examine the influence of minimum essential medium (MEM) vitamins supplementation to in vitro maturation medium and in vitro culture medium on the development of porcine e...
The Effect of the Addition and Removal of Various Cryoprotectants on the Nuclear Maturation and ATP Content of Immature Porcine Oocytes
Cryoprotectant Porcine Oocytes ATP Content
2016/4/29
This study was undertaken to investigate the influence of exposure and removal of four different cryoprotectants (CPAs) on the ATP content of cumulus cell-enclosed (COs) and cumulus cell-denuded (DOs)...
Effects of β-Mercaptoethanol on the Growth of Preantral Follicles and the Maturation of Intrafollicular Oocytes
Preantral Follicle In-vitro Culture b-Mercaptoethanol Follicle Growth Oocyte Maturation
2016/4/28
This study was undertaken to evaluate how -mercaptoethanol (bME), an exogenous antioxidant, interacts with preantral follicles cultured in vitro. Mouse primary or secondary follicles were cult...
Association between HSP70 Genotypes and Oocytes Development on In vitro Maturation/Fertilization in Pig
Fertilization Maturation Porcine Oocytes HSP70 Genotypes
2016/4/28
This study was performed to clarify whether the variation of stress related heat shock protein 70 (HSP70) (GenBank X68213) gene was associated with the nuclear morphological change of in vitro maturat...
An Antisense Oligodeoxynucleotide to the LH Receptor Attenuates FSH-induced Oocyte Maturation in Mice
Cumulus-enclosed Oocyte LH Receptor Oligodeoxynucleotide Mouse
2016/4/28
It has been recently shown that expression of the LH receptor (LHR) in cumulus cells is related with FSH-induced meiotic resumption of mouse cumulus enclosed oocytes (CEOs). However, to date, it is st...
Effects of Collection Methods on Recovery Efficiency, Maturation Rate and Subsequent Embryonic Developmental Competence of Oocytes in Holstein Cow
Recovery Efficiency Oocyte Maturation Rate Embryonic Developmental Competence
2016/4/25
Holstein cow ovaries obtained at a slaughterhouse were used to study the influence of the oocyte collection methods (slicing, puncture, aspiration I and II) on recovery efficiency and subsequent in vi...
Vascular Endothelial Growth Factor Has Beneficial Effect Independent of Serum Components throughout Oocyte Maturation and Early Embryonic Development in Cattle
Bovine Embryo Early Development IVM-IVF-IVC Serum VEGF
2016/4/21
In our previous studies, we demonstrated that Vascular Endothelial Growth Factor (VEGF) enhances bovine oocyte maturation and early embryonic development in serum supplemented media. In this experimen...
Gonadal Maturation and Spawning in Penaeus semisulcatus de Hann, 1844 by Hormone Injection
Penaeus semisulcatus maturation spawning, hormone injection eggs
2010/3/29
The effects of 3 hormones, HCG, LH-RH and serotonin (5-HT), on maturation and spawning of Penaeus semisulcatus de Hann, 1844 were investigated in 2 experiments in this study. The experiments were carr...
The Suppression of Maturational Competence by Streptomycin during In vitro Maturation of Goat Follicular Oocytes
Antibiotics Streptomycin In vitro Maturation Goat Oocytes
2016/4/15
Antibiotics are common additives in culture media during in vitro embryo development, but their effects on oocyte maturation in vitro have not been tested. The effects of penicillin, streptomycin and ...
MAP Kinase is Activated dring the Maturation of Porcine Oocytes
Porcine Oocytes Maturation Western Blotting MAP Kinase Phosphorylation
2016/4/15
In an attempt to evaluate the function of MAP kinase in porcine oocytes and to develop a method of the assessment of its activity, myelin basic protein (MBP) was used as a substrate to detect the MAP ...
Expression of the Antioxidant Enzyme and Apoptosis Genes in In vitro Maturation/In vitro Fertilization Porcine Embryos
Pig IVM/IVF Embryo RT-PCR Antioxidant Apoptosis
2016/4/14
This study was aimed at testing the gene expression of antioxidant enzymes and apoptosis genes for in vitro culture in porcine embryos produced by in vitro maturation/in vitro fertilization (IVM/IVF)....