搜索结果: 1-15 共查到“食品科学技术 DNA”相关记录24条 . 查询时间(0.25 秒)
限制性内切酶可以识别特定的脱氧核苷酸序列，并对DNA特定部位的两个脱氧核糖核苷酸之间的磷酸二酯键进行切割，被认为是自然界最精细的生物酶，是分子克隆技术的基石。近日，我校食品学院胥传来教授研究团队率先发现了手性纳米粒子的DNA特异性剪切效应，并实现了细胞与活体内靶标DNA的精确剪切。相关成果以“Site-Selective Photoinduced Cleavage and Profiling of...
Erratum for Zyba et al. A moderate increase in dietary zinc reduces DNA strand breaks in leukocytes and alters plasma proteins without changing plasma zinc concentrations
Zyba et al zinc DNA strand leukocyte
2018/11/21
Erratum for Zyba et al. A moderate increase in dietary zinc reduces DNA strand breaks in leukocytes and alters plasma proteins without changing plasma zinc concentrations. Am J Clin Nutr 2017;105: 343...
Only DNA-based dietary advice improved adherence to the Mediterranean diet score
DNA-based dietary Mediterranean diet score nutrition
2018/11/13
Livingstone et al. (1) examined the effects of various levels of personalized nutrition (PN) advice on adherence to a Mediterranean diet [Mediterranean diet (MedDiet) score]. Individuals were randomly...
A moderate increase in dietary zinc reduces DNA strand breaks in leukocytes and alters plasma proteins without changing plasma zinc concentrations
antioxidant DNA repair inflammation zinc biomarkers zinc fortification
2018/11/12
Background: Food fortification has been recommended to improve a population’s micronutrient status. Biofortification techniques modestly elevate the zinc content of cereals, but few studies have repor...
Postprandial alterations in whole-blood DNA methylation are mediated by changes in white blood cell composition
DNA methylation food intake ghrelin HumanMethylation450k EWAS
2018/12/13
Background: DNA methylation is an essential nuclear process associated with genomic functions such as transcription factor binding and the regulation of gene expression. DNA methylation patterns can a...
食源性致病菌DNA的数量及纯度严重影响后续分子生物学检测的准确性和灵敏性,因此随着分子生物学技术在食源性致病菌检测中的应用日渐广泛,细菌的分离、富集和DNA提取方法成为研究热点之一。本文综述了食源性致病菌的分离、富集和DNA提取的多种方法,以便研究开发更加快速、灵敏的分子生物学检测技术。
莴苣中两种致泻性大肠杆菌的富集及DNA提取方法的优化
鲜活农产品 致泻大肠杆菌 富集 DNA提取
2016/5/27
本研究对人工接种肠出血性大肠杆菌O157∶H7和肠侵袭性大肠杆菌的莴苣样品中菌体的富集和DNA提取方法进行了优化。研究中比较了不同过滤膜组合对菌体的富集效果,筛选了莴苣样品中细菌DNA提取的最适方法,建立了多重PCR方法检测人工污染莴苣样品。结果表明,采用尼龙膜15μm+混合膜0.22μm的组合富集细菌、试剂盒法提取样品中细菌DNA,多重PCR检测的检出限降低10倍,检测时间节省1.5 h。本研究...
芡实多糖的提取、抗氧化活性及对质粒DNA氧化损伤防护作用的研究
芡实 提取 抗氧化活性 DNA氧化损伤
2016/6/27
采用纤维素酶超声辅助法提取芡实多糖，由正交实验获得芡实多糖的最佳提取条件，并探究其体外抗氧化活
性及对] CW光解反应诱导质粒pBR322 D N A 损伤的保护作用。结果表明芡实多糖的最佳提取条件为：料液比1:20
(g/mL)、pH3、纤维素酶用量为原料质量的2.0%、超声时间5min、提取时间3h、提取温度4 0 1 ，多糖得率为17.04% 。
芡实多糖清除DPPH•、羟...
不同热处理方法对鲤鱼DNA的影响及不同DNA提取方法的比较
DNA提取方法 荧光定量聚合酶链反应
2015/12/16
研究不同加热方法处理后鲤鱼基因组DNA长度和含量的变化，并对4种DNA提取方法进行比较。方法 用电磁炉和微波炉将鲤鱼背部肌肉加热煮沸1、2、3 h取出，再分别用酚氯仿法、Wizard? 试剂盒法、磁珠法和离心柱法提取处理前后鲤鱼基因组DNA，利用琼脂糖凝胶电泳对处理前后的总DNA长度进行比较，再用实时荧光定量PCR法检测所得DNA样品中内参基因β-actin含量的变化。结果 琼脂糖凝胶电泳结果显示...
Utilization of DNA microarrays for detection and identification of selected Fusarium species from the Czech Republic
DNA microarrays fluorescent labelling Fusarium oligonucleotide probe PCR
2014/2/26
Fusarium is a serious phytopathogenic fungal genus with producting of many kinds of highly toxic secondary metabolites – mycotoxins. The consumption of Fusarium contaminated food and feed can cause da...
In this study methods of fungal DNA isolation were optimised and compared. The aim of the isolation processes was to obtain DNA of sufficient quality and quantity necessary for its amplification, as m...
Combined effects of temperature, pressure and low pH on the amplification of DNA of plant derived foods
GMO food processing DNA degradation DNA amplification
2014/2/25
The effect of food processing on the DNA integrity was studied by means of PCR amplification of soybean, transgenic MON 810 and non-transgenic maize, bean, and pea. The degree of DNA degradation was c...
Novel Bacteriocinogenic Lactobacillus plantarum Strains and Their Differentiation by Sequence Analysis of 16S rDNA, 16S-23S and 23S-5S Intergenic Spacer Regions and Randomly Amplified Polymorphic DNA Analysis
bacteriocin gene differentiation Lactobacillus plantarum ribosomal intergenic spacer region
2011/2/24
Six strains of bacteriocinogenic Lactobacillus plantarum (TL1, RG11, RS5, UL4, RG14
and RI11) isolated from Malaysian foods were investigated for their structural bacteriocin
genes. A new combinatio...
From DNA Sequences to Chemical Structures – Methods for Mining Microbial Genomic and Metagenomic Data Sets for New Natural Products
polyketides non-ribosomal peptides Actinobacteria homologous recombination
2010/10/14
Rapid mining of large genomic and metagenomic data sets for modular polyketide
synthases, non-ribosomal peptide synthetases and hybrid polyketide synthase/non-ribosomal
peptide synthetase biosynthet...
Protective Effects of Melanoidins Derived from Soy Sauce and Soy Paste on NO-Induced DNA Damage
melanoidin DNA damage comet assay
2009/6/17
We determined the protective activity of melanoidins against DNA damage using the comet assay. We used nitric oxide (NO) as a DNA damaging agent and HL60 (human promyelocytic leukemic cell line). Mela...