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The potential of Streptomyces as a probiotic feed for the growth of ornamental fish Xiphophorus helleri has been investigated. The Streptomyces strains used as probiotics were isolated from the marine...
The expression of bacterial polyketide synthase gene clusters is often controlled by a number of different families of regulatory proteins that can have either a pathway-specific or a pleiotropic mode...
Aminopeptidases (APs) of the same substrate specificities have been detected in the mycelia and culture filtrate of Streptomyces rimosus. To compare extracellular and intracellular prolyl, leucyl and ...
运用透明圈法从土样中筛选出产木聚糖酶放线菌80余株。对其中一株产酶情况较稳定的链霉菌菌株F0107进行了液体发酵的碳源、氮源、初始pH值、摇床转速及培养温度等条件的优化。实验表明,此菌株最适碳源为玉米芯水不溶性木聚糖,最适氮源为酵母浸膏与蛋白胨的复合氮源,最适初始pH值为5.0,最适产酶温度和最适摇床转速分别为32℃和140r/min。质量分数为0.4%的吐温80使菌株木聚糖酶产量提高41%,发酵...
An α-L-arabinofuranosidase gene of Aspergillus oryzae was expressed in Pichia pastoris. The re-combinant enzyme released L-arabinose from arabinose-containing polysaccharides such as lupin pectic gala...
The microbial transglutaminase (TGase) from Streptomyces libani was purified from its culture broth and its enzymatic properties were compared with those of TGase from Streptoverticillium mobaraense v...
An α-galactosidase gene belonging to glycoside hydrolase family 27 from Streptomyces coelicolor was cloned and expressed in E. coli. The purified enzyme showed a single protein band on SDS-PAGE with a...
The expression of seven tRNA genes from Streptomyces rimosus, cloned on bifunctional plasmids, has been studied in a homologous (S. rimosus) and a heterologous (Escherichia coli) system. Analyzed gene...
Electroduction is a method which allows direct transfer of plasmid DNA between donor and recipient under conditions of electroporation. The application of this technique allows significantly faster an...
One of the six Streptomyces rimosus ribosomal RNA operons, rrnF, was completely sequenced. rRNA genes, arranged in the order 16S-23S-5S, encode 1529, 3121 and 120 nucleotides long rRNAs, respectively....
Streptomyces rimosus lipase gene has been overexpressed in a heterologous host, S. lividans TK23. The maximal lipase activity was determined in the culture filtrates of the late stationary phase. Time...
Streptomycetes are important antibiotic producing bacteria that often exhibit genetic instability. One or both ends of the linear Streptomyces chromosome are lost spontaneously, resulting in viable mu...
Production of different pectinolytic enzymes was attempted using the actinomycete strain Streptomyces lydicus in submerged fermentation. Polygalacturonase and pectin lyase activities were detected in ...
Soybean protein isolate (SPI) was used in the investigation of the formation of edible protein films through an enzymatic cross-linking method with a purified microbial transglutaminase (MTG) produced...
α-Galactosidase production by a novel actinomycete strain Streptomyces griseoloalbus in shake flask culture was optimized using response surface methodology. Screening of variables to find their relat...

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