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Integrative Expression of Glucoamylase Gene in a Brewer’s Yeast Saccharomyces pastorianus Strain
glucoamylase brewer’s yeast diacetyl fermentation
2009/2/23
The recombinant brewer’s yeast Saccharomyces pastorianus strain was constructed by introducing the ilv2::GLA fragment released from pMGI6, carrying glucoamylase gene (GLA) and using the yeast α-acetol...
Site-Directed Mutagenesis of Tryptophan 622 of Thermoactinomyces vulgaris R-47 Glucoamylase:pH Optima and Activities of Five Mutants
glucoamylase optimal pH catalytic base
2008/4/20
In Aspergillus awamori glucoamylase, the optimal pH has been reported to increase to maintain activity by a mutation of Ser411 which forms a hydrogen-bond with a catalytic base (Fang and Ford, Protein...