搜索结果: 1-15 共查到“畜牧学 PCR”相关记录51条 . 查询时间(0.25 秒)
本试验旨在通过快速定量不同发酵时期下发酵豆粕中解淀粉芽孢杆菌（Bacillus amyloliquefaciens）的数量，以解决豆粕发酵过程品质监控的瓶颈难题，建立解淀粉芽孢杆菌的实时荧光定量PCR方法。根据解淀粉芽孢杆菌DNA解旋酶A亚基（gyrA）基因及16S核糖体RNA（16S rRNA）基因的保守区设计出3对引物，利用常规PCR筛选出1对特异性引物，并以该引物的扩增产物构建的重组质粒作为...
应用多重实时荧光PCR方法对8种动物毛纤维种类鉴别的研究
动物毛纤维 多重实时荧光PCR 鉴定
2018/7/26
本研究旨在利用多重实时荧光PCR方法对水貂、狐狸、骆驼、兔、山羊、绵羊、鸭和鹅8种动物毛纤维进行物种鉴定研究。利用碱裂解法加入二硫苏糖醇，并结合硅珠吸附核酸法来优化动物毛纤维DNA的提取；根据8种动物16S rDNA基因特异核苷酸序列设计通用PCR引物和特异性TaqMan荧光探针；通过优化引物和探针浓度及退火温度等PCR反应体系和条件，建立了多重实时荧光PCR检测体系；并对多重PCR体系的特异性、...
本研究对5种常用“持家基因”作为鸡肌肉组织发育早期基因和蛋白检测内参基因的有效性进行了评价，为鸡肌肉发育相关分子检测中内参基因的选择提供科学依据。应用实时荧光定量PCR（RT-qPCR）和Western blot技术，分别检测了鸡12胚龄、17胚龄、1日龄和14日龄胸肌中β肌动蛋白（ACTB）、β微管蛋白（TUBB）、TATA结合蛋白（TBP）、甘油醛-3-磷酸脱氢酶（GAPDH）和热休克蛋白70...
Differentiation of porcine wild-type lactobacilli strains, with ERIC-PCR and PFGE band patterns included in polyphasic taxonomy
Lactobacillus porcine lactobacilli phenotypic characterization genotypic characterization
2015/6/8
Probiotic administration is an alternative to antibiotic supplementation in pig management. However, potential probiotic microorganisms included in foods must be well defined and correctly named, on t...
Evaluation of the microbial population in ruminal fluid using real time PCR in steers treated with virginiamycin
virginiamycin roll-tube technique ruminal fermentation parameters real-time PCR
2015/6/8
The objective of the present study was to investigate the effects of virginiamycin (VM ) supplementation on ruminal fermentation and microbial populations in steers. Four ruminally cannulated Chinese ...
Simplified PCR analysis of a mutation in the NHEJ1 gene causing collie eye anomaly in some dog breeds
CEA polymorphism indel PikoTM Thermal Cycler allele frequencies
2015/6/8
Collie eye anomaly (CEA) is an inherited eye disease affecting development of the choroids and sclera segregating in several, mostly herding breeds of dog. Phenotypic development of the disease varies...
A relationship between the PCR-RFLP polymorphism in porcine MYOG, MYOD1 and MYF5 genes and microstructural characteristics of m. longissimus lumborum in Pietrain × (Polish Large White × Polish Landrace) crosses
MyoD genes muscle fibres pig
2015/6/5
Muscle fibre formation takes place during embryonic development and is regulated by the MyoD gene family, which consists of four genes, MYOD1, myogenin, MYF5 and MRF4. A relationship was studied betwe...
A simplified duplex real-time PCR incorporating TaqMan minor groove binder (MGB) probes and an exogenous internal positive control for the simultaneous detection of Mycoplasma gallisepticum and Mycoplasma synoviae cultures
avian Mycoplasma spp duplex real-time PCR
2015/5/25
Mycoplasma gallisepticum (MG) and Mycoplasma synoviae (MS) are the most pathogenic and
economically important mycoplasma pathogens that infect chickens. The development of rapid and innovative
mol...
Quantification of avian influenza virus in tissues of mute swans using TaqMan real time qRT-PCR
avian influenza virus qRT-PCR TagMan probe
2015/4/1
This study reports on the first quantification of avian influenza virus in the organs of mute swans that died during the epizootic of avian influenza (H5N1) between January and April 2006 in the Czech...
Identification of Pork Contamination in Meatballs of Indonesia Local Market Using Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) Analysis
Pork Contamination Detection Meatballs Polymerase Chain Reaction
2016/5/17
This research applied and evaluated a polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) using cytochrome b gene to detect pork contamination in meatballs from local markets...
为了建立同步检测奶牛布鲁菌、鹦鹉热衣原体和贝纳氏柯克斯氏体感染的多重PCR方法，根据GenBank上具有属间特异性的布鲁菌Bp26基因、鹦鹉热衣原体23S rRNA基因和贝纳氏柯克斯氏体IS1111a基因，利用 Primer premier 5.0软件各设计1对特异性引物。通过优化PCR反应体系和扩增条件，建立了能够同时检测奶牛布鲁菌、鹦鹉热衣原体和贝纳氏柯克斯氏体的多重PCR方法。该方法具有较好...
本试验旨在采用聚合酶链式反应-变性梯度凝胶电泳(PCR-DGGE)技术分析瘤胃菌群在不同纤维素富集条件下的多样性。试验采用羧甲基纤维素(CMC)培养基、蛋白胨纤维素(PCS)培养基、J培养基和K培养基分别在37和50℃条件下培养瘤胃内容物,使用PCR-DGGE技术、DGGE图谱共性及特异性条带的克隆和测序、聚类分析和主成分分析(PCA)几种方法分析瘤胃菌群多样性。结果表明:37℃条件下,不同培养基...
本研究旨在建立多重Real-time PCR方法检测猪繁殖与呼吸综合征病毒(PRRSV)、猪圆环病毒2型（PCV2）、猪伪狂犬病毒（PRV）和猪细小病毒(PPV)。根据GenBank数据库中PRRSV、PCV2、PRV、和PPV的核苷酸序列设计特异性引物和探针，通过优化反应条件，检测系列稀释的纯化阳性质粒，建立检测PRRSV、PCV2、PRV和PPV的单项和多重Real-time PCR方法。灵敏...
本试验旨在研制小鹅瘟和雏鹅新型病毒性肠炎二联PCR检测试剂盒,观察临床应用效果。根据小鹅瘟和鹅病毒性肠炎基因序列,设计合成一对特异性引物,对PCR反应条件进行优化,并分别对试剂盒的特异性、敏感性、重复性和保存期进行研究,之后进行临床扩大试验。该二联PCR诊断试剂盒能扩增出预期的小鹅瘟375 bp核酸片段和鹅病毒性肠炎223 bp核酸片段,与鸭瘟病毒、鹅副黏病毒无交叉反应;最低能检测到小鹅瘟病毒10...
使用poly(A)-tailed RT-PCR对猪部分microRNAs在胚胎期的表达进行验证
poly(A)-tailed RT-PCR 猪 microRNAs 胚胎期 进行验证
2011/8/31
MicroRNAs(miRNAs)是一系列小的非编码的RNA，长度大约为21~22 nt。主要通过抑制翻译或者进行mRNA的降解而在多种生理以及发育过程中发挥很重要的作用miRBase16.0(2010)数据库中显示，注释的线虫﹑小鼠﹑大鼠以及人的miRNAs分别有175﹑673﹑408和1048种。而哺乳动物猪的miRNAs仅仅为211种，特别是在胚胎生长发育过程中调节基因表达的miRNAs的研...