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Lignosulfonic acid promotes hypertrophy in 3T3-L1 cells without increasing lipid content and increases their 2-deoxyglucose uptake
Adipogenesis Diabetes Dietary Fiber Glucose Uptake Lignosulfonic Acid
2017/3/7
Adipose tissue plays a key role in the development of obesity and diabetes. We previously reported that lignosulfonic acid suppresses the rise in blood glucose levels through the inhibition of α-gluco...
Vitamin A affects the expression of antioxidant genes in bovine mammary epithelial cells with oxidative stress induced by diethylene triamine-nitric oxide
nitric oxide antioxidant enzymes inflammatory factors
2016/3/24
The considerable increase in oxygen requirements due to the high metabolic rate of the bovine mammary epithelial cells (BMEC) during lactation results in an augmented production of reactive nitrogen s...
Effect of the ratios of acetate and β-hydroxybutyrate on the expression of milk fat-and protein-related genes in bovine mammary epithelial cells
ratio of short chain fatty acids milk fat precursor dairy cow milk fat milk protein gene expression
2015/12/17
The objective of this study was to evaluate the effects of the different ratios of acetate and β-hydroxybutyrate (BHBA) on cell viability, triacylglycerol (TAG) content, and mRNA expression of the gen...
Gene expression of six major milk proteins in primary bovine mammary epithelial cells isolated from milk during the first twenty weeks of lactation
dairy cow immunomagnetic cell separation
2015/6/23
Using this method, it was tested whether the
number of pBMEC and the relation of recovered pBMEC to total somatic cell count vary within the individual
lactation stages. Furthermore, the expressio...
Aberrant cytoplasmic accumulation of retinoblastoma protein in basal cells may lead to increased survival in malignant canine mammary tumours
canine mammary tumour dogs immunohistochemistry retinoblastoma protein
2015/4/17
The retinoblastoma susceptibility gene RB-1 is a tumour suppressor gene that encodes a protein (Rb) that regulates the transition from the G1 phase to the S phase of the cell cycle. Inactivation of th...
The effect of swim-up purification and incubation of cells on sperm viability in dogs of different ages
dogs sperm sperm viability necrosis flow cytometry
2015/4/15
The influence of selected semen extenders on the motility of frozen-thawed dog spermatozoa has been clearly demonstrated in several studies, although there are no reports indicating the effect of swim...
Lactoferrin inhibits E. coli O157:H7 growth and attachment to intestinal epithelial cells
transferring type III secretion system EspA
2015/4/7
Enterohemorrhagic Escherichia coli (EHEC) serotype O157:H7 strains are associated with haemorraghic colitis and haemolytic uremic syndrome (HUS) in humans. Cattle are a reservoir of E. coli O157:H7. W...
Radiosensitivity of peripheral blood B cells in pigs
dosimetry-biological B lymphocytes radiosensitivity
2015/4/2
Swine are here introduced to biodosimetry in an attempt to develop a large animal model allowing for comparison of in vitro experiments with the in vivo processes occurring after exposure to gamma rad...
Genomic and non-genomic effects of progesterone and pregnenolone on the function of bovine endometrial cells
non-genomic effect progesterone progesterone membrane receptor component 1
2015/4/2
Progesterone (P) decreases oxytocin (OT)-stimulated prostaglandin (PG)F2α, but not PGE2 secretion from bovine endometrial cells and this effect is partly elicited via a non-genomic route. The aim of t...
Dose-dependent immune response in milk cells and mammary tissue after intramammary administration of lipopolysaccharide in dairy cows
mammary gland mastitis lipopolysaccharide cattle
2015/3/13
The immune response in milk cells and the status of mammary tight junctions (TJ) in response to intramammary (IM) infusion of different doses of Escherichia coli lipopolysaccharide (LPS) was investiga...
Effect of polychlorinated biphenyls on the secretion of oxytocin from luteal and granulosa cells in cow: possible involvement of glucocorticoid receptors
polychlorinated biphenyls oxytocin glucocorticoid receptors ovary cow
2015/3/5
Polychlorinated biphenyls (PCBs) stimulate oxytocin secretion from bovine granulosa and luteal cells. Since oxytocin on the one hand is released from ovarian cells by cortisol and on the other hand PC...
Immune relevant gene expression of mammary epithelial cells and their influence on leukocyte chemotaxis in response to different mastitis pathogens
infection mammary gland Escherichia coli Staphylococcus aureus Streptococcus uberis
2015/3/5
Different mastitis pathogens induce different courses of infection, i.e. more or less severe. Mammary epithelial cells play an important role in the initial combat against microorganisms by expression...
Appearance of iron-labeled blood mononuclear cells in electron microscopy
ultrastructure iron nanoparticles cultivation animal model
2015/3/5
Mononuclear cells from rabbit bone marrow were cultured for 14 days in cell-free medium for hematopoietic cells together with iron oxid nanoparticles, and then they were processed by technique for fre...
Effect of polychlorinated biphenyls (Aroclor-1248) on the secretory function of bovine luteal cells affected by LH, noradrenaline and high density lipoproteins
bovine PCBs corpus luteum progesterone oxytocin HDL
2015/1/27
The corpus luteum (CL), formed from the ruptured follicle, is required for the course of normal cyclicity and the duration of pregnancy in females. The influence of a mixture of polychlorinated biphen...
Detection of neoplastic cells in blood of miniature pigs with hereditary melanoma
MeLiM strain melanoblastoma
2015/1/20
Tyrosine, a key enzyme of melanin biosynthesis, is widely used as a specific marker for the detection of dissemination or metastatic melanoma cells in peripheral blood and other tissues like lymph nod...