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旨在细胞水平上进一步探讨FUT2基因的功能及其在猪小肠上皮细胞受到F18大肠杆菌（Escherichia coli，E.coli）侵染时的调控机制，为提高仔猪对F18大肠杆菌病抵抗力的分子选育提供理论基础。运用Real-time PCR方法检测分析FUT2基因在大肠杆菌F18ab、F18ac感染和脂多糖（LPS）诱导小肠上皮细胞前后的mRNA表达差异，并采用Western blotting方法检测...
Molecular characterisation of Shiga toxin-producing Escherichia coli O157:H7 isolates from cattle in eastern Turkey
pulsed-field gel electrophoresis virulence genes bovine rectal swabs E. coli strains
2016/12/8
Shiga toxin-producing Escherichia coli (STEC) O157:H7 is an important foodborne pathogen worldwide. In Turkey, little is known about the molecular characteristics of STEC O157:H7 isolates. The aim of ...
Antimicrobial effects of curcumin against L. monocytogenes,S.aureus,S.Typhimurium and E.coli O157:H7 pathogens in minced meat
curcumin antimicrobial effect foodborne pathogen minced meat
2016/6/3
The aim of this study was to determine the antimicrobial efficacy of curcumin, one of the active components of the Curcuma longa (turmeric) plant, against food pathogens in a minced meat medium. Salmo...
Effects of a species-specific probiotic formulation on multiresistant Escherichia coli isolates from the gut of veal calves
lactic acid bacteria antibiotic resistance prevalence gastrointestinal functionality veal calves
2015/6/24
In this study, 254 Escherichia coli isolates from faecal samples of veal calves were evaluated for antimicrobial susceptibility using the disk diffusion method. During the experimental period, six mas...
Atresia coli in a Japanese black calf diagnosed by a barium sulphate enema contrast radiograph in the standing position:a case report
atresia coli contrasts radiography enema Japanese black calf standing position
2015/4/16
A three day-old Japanese black calf was admitted with a history of abdominal distension and absence of defecation. Dilated loops of the small intestine and hypoplasia of the colon and rectum was obser...
The survival of Escherichia coli upon exposure to irradiation with non-coherent polychromatic polarized light
bacteria growth light plate count
2015/4/15
The purpose of this work was to examine the influence of non-coherent polarized light upon the growth of Escherichia coli. Experiments were designed to test the main hypothesis that this kind of polyc...
Susceptibility of Escherichia coli, Salmonella sp. and Clostridium perfringensto organic acids and monolaurin
fatty acids monolaurin enteropathogenic bacteria inhibition
2015/3/5
The antimicrobial activity of fatty acids, monolaurin, citric, succinic, fumaric, malic and lactic acid was determined in cultures of two strains of Escherichia coli, three strains of Salmonella sp. a...
Survival of Escherichia coli O157:H7, Listeria monocytogenes 4b and Yersinia enterocolitica O3 in ayran and modified kefir as pre- and postfermentation contaminant
ayran modified kefir E. coli O157
2015/1/28
The survival of Escherichia coli O157:H7, Listeria monocytogenes 4b and Yersinia enterocolitica O3 in traditional yogurt and kefir during fermentation, in ayran (a dairy beverage in Turkey), pasteuris...
CD45RA and CD45RC isoforms expression in weaned pigs vaccinated with non-enterotoxigenic F4ac+ Escherichia coli strain against colibacillosis
weaned pigs vaccination colibacillosis CD45 isoforms CD25 flow cytom
2015/1/26
Since no effective vaccine is available for its immunoprophylaxis, porcine post-weaning diarrhoea (PWD) induced by enterotoxigenic Escherichia coli (ETEC) strains remains an important cause of morbidi...
Gene typing of the colonisation factors F18 of Escherichia coli isolated from piglets suffering from post-weaning oedema disease
oedema disease pig colonisation factors Stx2e toxin PCR
2015/1/26
Production of verotoxin Stx2e and expression of F18 and K88 colonisation factors were investigated in 222 strains of Escherichia coli isolated from weaned piglets. Sixty-two, 30, and 11 of the 129 ver...
Molecular analysis of Escherichia coli O157 strains isolated from cattle and pigs by the use of PCR and pulsed-field gel electrophoresis methods
E. coli O157 cattle pigs virulence marker genes RFLP genetic relatedness
2015/1/26
Fourteen Escherichia coli O157 strains isolated from cattle and pigs in Poland and in Germany were investigated, using PCR, for the genetic markers associated with Shiga toxin-producing E. coli (STEC)...
梅山猪E.coli F18菌株攻毒试验及其表型的鉴定分析
猪 腹泻 F18大肠杆菌 攻毒试验
2014/12/25
利用E.coli F18菌株攻毒试验来获得梅山猪断奶仔猪E .coli F18敏感和抗性型个体，可以为今后利用高通量技术研究仔猪抗E .coli F18遗传机理提供宝贵的素材，同时也为中国地方猪品种细菌性疾病模型的建立提供指导和参考。本研究以中国地方品种梅山猪断奶仔猪为研究对象，采用F18ab和F18ac菌株口服攻毒试验，并利用仔猪肠道E.coli F18细菌检测、细菌计数以及小肠上皮细胞黏附试验...
The use of artificial intelligence (artificial neural networks) to classify the biochemical reactions of Escherichia coli isolates from broilers
Escherichia coli biochemical reactions artificial neural network
2010/9/21
The Escherichia coli is often found in the poultry industry and, many times, its presence in the organism of the animals and/or contaminating the litter of poultry houses don’t cause surprise. On the ...
Profiles of Virulence-associated Genes of Avian Pathogenic Escherichia coli Isolates from Chickens with Colibacillosis
APEC broilers molecular detection virulence genes
2009/7/29
The colibacillosis caused by avian pathogenic E.coli (APEC) is responsible for a significant loss of productivity and mortality in the poultry industry. The pathogenicity of these bacteria is based on...
不同猪种 E.coli F18受体基因的多态性
猪 E.coli F18受体基因 PCR-RFLP 多态性
2008/1/21
采用PCR-RFLP技术检测了大约克、长白、杜洛克、宁乡、沙子岭和大围子6个品种共867头猪的E.coli F18受体(ECF18R)基因座的遗传变异。结果表明：Hin6Ⅰ-RFLP位点上，大约克、长白、杜洛克3个外来猪种均存在多态,且以敏感型（GG型和AG型）居多，平均占94%，3个外来猪种的G等位基因频率平均为0.76，AA抗性型个体占少数，平均为6%，猪群中M307处G→A的突变频率并不高。...