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The role of cyclooxygenase inhibitors in lipopolysaccharide-induced hypophagia in chicken
prostaglandins cytokines food intake broiler
2015/9/15
Previous studies showed that cyclooxygenase 1 (COX) enzyme has an important role in lipopolysaccharide (LPS)-induced hypophagia in mammals but the effect of COX on LPS-induced hypophagia has not been ...
In vitro susceptibility of duck, chicken, and pig erythrocyte lipids to peroxidation
duck chicken pig erythrocytes thiobarbituric acid-reactive substances glutathione
2015/1/26
The susceptibility of erythrocyte lipid to in vitro peroxidation as measured by TBARS (thiobarbituric acid-reactive substances) and concentration of reduced glutathione (GSH) in whole blood and erythr...
Identification of Transferred Chicken Germ Cells in Quail Gonad and Semen by Amplification of Chicken-Specific PCR Products
microsatellite DNA chicken PCR primordial germ cells quail
2009/8/3
A polymerase chain reaction (PCR) primer set for a chicken microsatellite locus on the Z chromosome, LEI0171 (GenBank Accession No.X85538), amplified a 363bp fragment of genomic DNA in chickens (Gallu...
Restoration of Genetic Resources from Ehime Native Chicken via Transferred Primordial Germ Cells (PGCs)
chimeras germ line primordial germ cell embryos
2009/8/3
This experiment was designed to produce chimeric chicken by transferring primordial germ cells (PGCs) of Ehime native chicken embryos to White Leghorn ones. Fertilized eggs produced by both chicken br...
Effect of Estradiol and Nonylphenol on mRNA Levels of VitellogeninII in the Liver of Chicken Embryos
nonylphenol estradiol vitellogenin II chicken embryo RT-PCR
2009/8/3
Nonylphenol is one of the endocrine disrupting agents with estrogenic activity in some vertebrates. The present study was conducted to assess estrogenic activity of p-nonylphenol (NP) in chicken embry...
Effect of Estradiol and Nonylphenol on mRNA Expression of Estrogen Receptors α and β,and Cytochrome P450 Aromatase in the Gonad of Chicken Embryos
p-nonylphenol ERα ERβ P450arom endocrine disruptor
2009/7/31
Effects of estradiol-17β (E2) and p-nonylphenol (NP) on mRNA expression of estrogen receptor α (ERα), estrogen receptor β (ERβ) and cytochrome P450 aromatase (P450arom) were examined in the early stag...
Efficient Gene Transfer into Early Chicken Embryos by Electroporation of Stage X Blastoderms in Vivo, Applying Electric Pulses Vertically to the Blastoderm Layer
blastoderm chick embryo electroporation embryo culture GFP gene
2009/7/31
In order to introduce exogenous DNA into early chicken embryos by transfecting the stage X (EG&K) blastoderm, in vivo electroporation was applied to it. A pair of novel electrodes was devised for appl...
The present experiment was designed to investigate a possibility of the in vitro cultured somatic cells to produce the germ line interspecific chimera in bird. The frozen-thawed fibroblast-like cells ...
Preparation of Monoclonal Antibody Against Chicken Apolipoprotein B and Development of Enzyme Liked Immunosolvent Assay (ELISA) Method with the Antibody Aiming at the Optimization of Lipid Metabolism in Chickens
apolipoprotein B monoclonal antibody enzyme linked immunosorbent assay fat deposition broiler chickens
2009/7/31
Apolipoprotein B (apoB) containing lipoproteins are major lipoproteins in blood and the elevated concentration in plasma may lead to increased transport of lipoproteins into adipose tissues resulting ...
Effect of Electrode Size on Transfection Efficiency of Chicken Stage X Blastoderm by in vivo Electroporation
blastoderm electrode electroporation GFP gene
2009/7/31
In order to improve transfection efficiency of the stage X blastoderm of chickens by in vivo electroporation, the effect of the size of the upper and lower electrodes was examined. The plasmid DNA (...
Dioxin Concentrations in Body Tissues and Egg of Female Chicken
accumulation body tissues chicken dioxins egg
2009/7/31
To investigate the transfer and accumulation of ingested dioxins, the concentra-tions of polychlorinated dibenzo-p-dioxins (PCDDs), polychlorinated dibenzofurans (PCDFs), and coplanar-polychlorinated ...
Effect of T-2 Toxin in Combination with Vitamin E,Selenium and Mycotoxin Binder on Lipid Peroxide Status and on the Glutathione Redox System in Broiler Chicken
glutathione glutathione-peroxidase lipid peroxidation T-2 toxin vitamin C
2009/7/30
The effect of T-2 toxin (2.35mg/kg of feed) on lipid peroxidation and on the antioxidant defence system was studied in blood plasma, red blood cells (RBC), liver and spleen of broiler chickens. The in...
Mitochondrial Oxidative Damage in Chicken Skeletal Muscle Induced by Acute Heat Stress
2D electrophoresis-protein carbonyl heat stress lipid peroxidation MDA oxidized protein
2009/7/30
Mitochondria have long been known to play a critical role in maintaining bioenergetic status under physiological conditions. Our previous studies have shown that acute heat stress increases production...
Structure and Tissue Distribution of Cholecystokinin-1 Receptor in Chicken
cDNA cloning chicken cholecystokinin receptor
2009/7/30
Chicken cholecystokinin-1 receptor (chCCK1R) cDNA was isolated from a chicken brain and tissue distribution of the chCCK1R mRNA was determined by RT-PCR. The chCCK1R is composed of 429 amino acid resi...
Microsatellite Analysis of Genetic Diversity in Indian Chicken Populations
chicken genetic distance genetic diversity India microsatellite markers
2009/7/30
Characterization of genetic diversity by employing molecular tools is a prerequisite in developing strategies for conservation and utilization of poultry genetic resources. We assessed genetic variati...