搜索结果: 1-15 共查到“基础医学 IgE”相关记录32条 . 查询时间(0.076 秒)
探讨儿童支气管哮喘严重程度与血清嗜酸粒细胞阳离子蛋白(eosinophil cationic protein，ECP)、总IgE(Total IgE，TIgE)、嗜酸粒细胞(eosinophils，EOS)水平的关系;了解儿童支气管哮喘常见过敏原，为儿童支气管哮喘的预防、病情判断及治疗提供依据。方法 据儿童哮喘严重程度分级标准将95例哮喘患儿分为间歇发作组(n=23)、轻度组(n=29)、中度组(...
Lipocalin-Type Prostaglandin D Synthase and Egg White Cystatin React with IgE Antibodies from Children with Egg Allergy
cystatin food allergy hen's egg white LC-MS/MS lipocalin-type prostaglandin D synthase
2010/11/12
Background: Ovalbumin, ovomucoid, ovotransferrin, lysozyme, and ovomucin are known to be major allergens found in egg white. Egg white protein is composed of over 30 proteins; many of which have neith...
Genetic Impact of a Butyrophilin-like 2 (BTNL2) Gene Variation on Specific IgE Responsiveness to Dermatophagoides farinae (Der f) in Japanese
BTNL2 Dermatophagoides farinae (Der f) specific IgE
2010/11/16
Controlling for gender, age, smoking, and the presence of asthma, multiple logistic regression analyses showed that homozygosity of the rs2076530 A allele, which has been reported to be a risk allele ...
虫源性IgE依赖组胺释放因子诱导致敏肥大细胞释放组胺的研究
日本血吸虫 华支睾吸虫 IgE依赖组胺释放因子 肥大细胞 组胺
2009/2/25
目的 制备日本血吸虫和华支睾吸虫IgE依赖组胺释放因子重组蛋白rSjHRF和rCsHRF，并观察两者诱导大鼠致敏肥大细胞释放组胺的功能。 方法 分别克隆SjHRF和CsHRF基因完整编码序列，将重组质粒分别转化大肠埃希菌BL21并诱导表达，亲和层析纯化可溶性表达的重组蛋白，将纯化的rSjHRF和rCsHRF分别与卵清蛋白变应原致敏的大鼠肺肥大细胞一起孵育，利用荧光分光光度法测定肥大细胞组胺释放...
目的 探讨细粒棘球蚴(E.g.)囊液粗制抗原和B抗原(EgB)识别绵羊感染E.g.后及诱发过敏性休克期间特异性IgG和IgE抗体的特异性反应,并对抗原特性及分子量进行描述。 方法 制备E.g.囊液粗制抗原及EgB,应用免疫印迹技术检测经剖杀证实感染E.g.并诱发过敏性休克的20只绵羊血清特异性IgG和IgE抗体对两种抗原的抗体反应性,并对抗原特性和分子量进行描述。 结果 特异性IgE抗体与耐热、低...
目的 从日本血吸虫成虫cDNA文库中筛选并克隆日本血吸虫特异性IgE相关抗原编码基因。 方法 用ABC ELISA法从日本血吸虫病流行区筛选出高水平抗血吸虫成虫抗原IgE抗体的个体 15人 ,采集血清并混合。混合血清经Protein G柱吸收后 ,用于日本血吸虫成虫cDNA文库的免疫学筛选。PCR扩增阳性克隆插入cDNA片段。序列分析后 ,于该序列第一开读框两端设计引物并分别引入EcoRⅠ和Not...
目的 纯化重组日本血吸虫特异性IgE抗体相关蛋白和鉴定其免疫原性。 方法 大量表达Sj43B/pGEX 6p 1 /BL2 1重组克隆菌 ,超声粉碎后离心获得融合表达的重组蛋白包涵体。经TNMFX缓冲液分步洗涤后 ,将包涵体溶解液经FPLC分离 ,获得重组融合蛋白组分 ,再经巯基二硫键转换复性后 ,用于免疫小鼠获得抗血清 ,分别用dot ELISA法和Westernblotting法对融合蛋白引起...
Applying Surface Plasmon Resonance to Monitor the IgE-Mediated Activation of Human Basophils
basophil biosensor histamine release IgE surface plasmon resonance
2010/11/17
When basophils were sensitized with antigen specific IgE, they immediately caused the increase of resonance angle (AR) in response to either anti-IgE antibody or corresponding antigens, even when they...
Genetic Variability of the High-affinity IgE Receptor α Subunit (FcεRIα) is Related to Total Serum IgE levels in Allergic Subjects
allergy FCER1A FcεRIα genetic polymorphism IgE omalizumab
2010/11/18
Known susceptibility genes to atopy and asthma have been identified by linkage or associations with clinical phenotypes, including total serum IgE levels. IgE-mediated sensitivity reactions require a ...
Osteopontin: A Potential Biomarker for Successful Bee Venom Immunotherapy and a Potential Molecule for Inhibiting IgE-mediated Allergic Responses
biomarker clustering mechanism microarray osteopontin (OPN) venom immunotherapy (VIT)
2010/11/19
Venom immunotherapy (VIT) is proven to be curative for insect allergy, but the mechanisms and the biomarkers associated with clinical efficacy remain elusive. We report herein the discovery of a leadi...
Association of the Expression of IL-4 and IL-13 Genes,IL-4 and IgE Serum Levels with Allergic Asthma
Asthma Genes IL-4 IL-13 RT-PCR
2010/4/9
Immune and inflammatory responses mediated by cytokines, play important roles in the
pathophysiology of asthma. These responses are associated with overexpression of Th2 cytokines
such as IL-4 and I...
Association of the Expression of IL-4 and IL-13 Genes,IL-4 and IgE Serum Levels with Allergic Asthma
Asthma Genes IL-4 IL-13 RT-PCR
2010/4/9
Immune and inflammatory responses mediated by cytokines, play important roles in the
pathophysiology of asthma. These responses are associated with overexpression of Th2 cytokines
such as IL-4 and I...
Characterization of Specific IgE Antibody Related to Antigen 5 of Echinococcus granulosus
Cystic Echinococcosis Antigen 5
2010/4/7
Background: Anaphylactic reactions, such as urticaria, edema, respiratory symptoms, and anaphylactic shock often complicate the course of Cystic Echinococcosis (CE). Methods: To investigate the role o...