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siRNA Targeted to p53 Attenuates Ischemic and Cisplatin-Induced Acute Kidney Injury
acute kidney injury siRNA
2014/12/11
Proximal tubule cells (PTCs), which are the primary site of kidney injury associated with ischemia or nephrotoxicity, are the site of oligonucleotide reabsorption within the kidney. We exploited this ...
通过siRNA(RNAi)干扰选择性下调TLR4基因的表达,探讨高糖对大鼠肾小管上皮细胞(NRK-52E)Toll样受体4(toll-like receptor 4,TLR4)及其转接子髓分化因子88(myeloid differentiation factor 88,MyD88)和炎性因子分泌的影响。 方法 设计并合成3对针对大鼠TLR4基因的特异性siRNA片段,以带有红色荧光的BLOC...
观察信号转导与转录激活子1(STAT1)siRNA转染后高糖培养人肾小球系膜细胞(HMC)的增殖情况及细胞内STAT1、磷酸化(p)STAT1、STAT3、p-STAT3蛋白及下游TGF-β1的表达变化,探讨高糖状态下STAT1和STAT3活性变化及STAT亚型变化对TGF-β1的影响。 方法 设计并合成针对STAT1基因的3个特异性siRNA序列(STAT1-siRNA),应用Lipofe...