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The sperm chromatin structure assay. Relationship with alternate tests of semen quality and heterospermic performance of bulls
sperm chromatin structure assay alternate tests semen quality heterospermic performance
2009/2/23
Data obtained by the sperm chromatin structure assay (SCSA) on spermatozoa from nine bulls were correlated with fertility, measured by heterospermic performance (-0.94, P less than 0.01) and by al...
Suppression of steroid receptor-chromatin interaction by inhibitors present in ammonium sulfate-fractionated rat testicular androgen receptor preparations
steroid receptor-chromatin interaction inhibitors present ammonium sulfate-fractionated rat
2009/2/23
Tritiated androgen receptor complex was partially purified by ammonium sulfate precipitation at 15 to 37% saturation from rat testicular or prostatic cytosols. A greater amount of prostatic tritiate...
Chromatin structural changes in sperm after scrotal insulation of Holstein bulls
Heat stress bovine sperm chromatin semen quality
2009/2/17
The reported effects on semen quality ascribed to testicular heat stress generally relate to traits impacting sperm transport and fertilizing ability but not to the genetic material contained by the...
Effects of heat stress on mouse testicular cells and sperm chromatin structure
Testicular heat stress SCSA
2009/2/17
Scrotal regions of mice were exposed to a 38.0, 40.0, or 42.0 degrees C (+/-0.1) H2O bath for 60 minutes to determine the effects of elevated temperatures on testicular cells and sperm chromatin str...
The nuclear status of human sperm cells by TEM image cytometry：nuclear shape and chromatin texture in semen samples from fertile and infertile men
human sperm cells TEM image cytometry nuclear shape chromatin texture semen samples
2009/2/12
Changes in nuclear size, shape, and chromatin texture during spermiogenesis and epididymal transport of human sperm were recently analyzed using transmission electron microscopy (TEM) image cytome...
Studies on sperm chromatin structure alterations and cytogenetic damage of mouse sperm following in vitro incubation. Studies on in vitro-incubated mouse sperm
sperm chromatin structure alterations cytogenetic damage of mouse sperm
2009/2/12
Mouse epididymal sperm incubated in Tyrode's T6 fertilization media were analyzed over time for chromosome damage by two methods. First, cytogenetic analysis was done on paternal pronuclei metapha...
Comparison between human sperm preservation medium and TEST-yolk buffer on protecting chromatin and morphology integrity of human spermatozoa in fertile and subfertile men after freeze-thawing procedure
Acridine orange chromatin normality cryopreservation
2008/12/29
The aim of this study was to identify the detrimental effect of the freeze-thaw process on chromatin integrity and morphology of human spermatozoa, and to determine whether human sperm preservation ...
Chromatin structure in globozoospermia：a case report
Chromatin structure globozoospermia case report
2008/12/26
Sperm nuclear abnormalities in patients with globozoospermia have not been well characterized and may lead to the high rates of fertilization failure and embryo loss reported in patients with this for...
Comparative study of cytochemical tests for sperm chromatin integrity
Comparative study cytochemical tests sperm chromatin integrity
2008/12/26
Tests were carried out on sperm from 40 fertile and infertile men to evaluate 2 DNA in situ denaturation methods using acridine orange (AO; the modified Rigler-Roschlau method and the Tejada method), ...
Characteristics of human sperm chromatin structure following an episode of influenza and high fever：a case study
Characteristics human sperm chromatin structure episode influenza high fever
2008/12/25
Semen samples from a fertile patient presenting with influenza and a 1-day fever of 39.9 degrees C were obtained and analyzed at 18-66 days postfever (dpf) for sperm nuclear proteins, DNA stainabi...
The Relationship Between Sperm Morphology and Chromatin Integrity in the Koala（Phascolarctos cinereus）as Assessed by the Sperm Chromatin Dispersion Test（SCDt）
DNA damage marsupial
2008/12/24
Koala (Phascolarctos cinereus) sperm nuclei show a tendency to swell after cryopreservation, but it is uncertain whether this phenomenon is associated with DNA fragmentation. In this study, we va...
Effects of the Chemotherapy Cocktail Used to Treat Testicular Cancer on Sperm Chromatin Integrity
Sperm DNA DNA strand breaks
2008/12/24
The incidence of testicular cancer has increased dramatically over the past 50 years. Advances in treatment, which include the coadministration of bleomycin, etoposide, and cis-platinum (BEP),...
Increased Aneuploidy Rate in Sperm With Fragmented DNA as Determined by the Sperm Chromatin Dispersion（SCD）Test and FISH Analysis
Human fragmentation
2008/12/24
Previous studies suggest that sperm DNA fragmentation may be associated with aneuploidy. However, currently available tests have not made it possible to simultaneously perform DNA fragmentatio...
Seasonal Changes in Sperm Chromatin Condensation in Ram（Ovis aries）, Iberian Red Deer （Cervus elaphus hispanicus）, and Brown Bear（Ursus arctos）
SCSA season
2008/12/24
The effect of seasonality (temperate environment, Spain) on the chromatin status of ovine (Churra breed), Iberian red deer, and brown bear spermatozoa was studied. This work aims to improve genet...
Infertile Men With Varicocele Show a High Relative Proportion of Sperm Cells With Intense Nuclear Damage Level, Evidenced by the Sperm Chromatin Dispersion Test
Human sperm DNA fragmentation
2008/12/23
The frequency of sperm cells with fragmented DNA was studied in a group of 18 infertile patients with varicocele and compared with those obtained in a group of 51 normozoospermic patients, 103 patie...