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Purification and characterization of a sperm motility inhibitor in human seminal plasma
sperm motility inhibitor human seminal plasma
2009/2/23
A sperm motility inhibitor from human seminal plasma was purified and characterized. The purification procedure includes dialysis, ion exchange chromatography on SP-Sephadex C-25 and adsorption chro...
Comparison between proteinases of human seminal plasma and of sperm origin
proteinases human seminal plasma sperm origin
2009/2/23
A comparison of the alkaline proteinase activity of human seminal plasma, the seminal non-gamete cellular material and spermatozoa was made by gelatin-sodium dodecyl sulfate-polyacrylamide gel elect...
Similar biochemical properties of human seminal plasma and epididymal alpha-1,4-glucosidase
1-4 glucosidase seminal plasma epididymis
2009/2/20
We have studied some characteristics of alpha-1,4-glucosidases in human male reproductive organs in order to obtain information on the origin of the enzyme in seminal plasma. Acid and neutral enzyme...
Follicle-stimulating Hormone Receptor Binding Inhibitors in Human Seminal Plasma
FSH binding inhibition seminal plasma
2009/2/19
Human seminal plasma, freed of spermatozoa by centrifugation, inhibited binding of iodine-125 (125I)-hFSH to receptors in calf testes. Binding inhibition (BI) was seen in seminal plasma from intact ...
Occurrence of DOPA/5-HTP Decarboxylase in Human Seminal Plasma：Kinetic Parameters and Demonstration of a Naturally Occurring Inhibitor of the Enzyme
DOPA/5-HTP decarboxylase human seminal plasma biogenic amines
2009/2/18
The authors found that DOPA/5-HTP decarboxylase was present in the human seminal plasma and that its activity was lower in fertile men than in infertile men. The Km and Vmax of the enzyme were, re...
Origin of an 84-kDa protein with ABH blood-typing antigen activity in human seminal plasma
p 84 ABO blood group substance lactotransferrin
2009/2/18
In order to investigate the origin of an 84-kDa protein with ABH blood-typing antigen activity (p 84) and its concentration in human seminal plasma, a monoclonal antibody (mAb p 84) was produced....
Immunoreactive parathyroid hormone-related protein is present in human seminal plasma and is of prostate origin
Immunoreactive parathyroid hormone-related protein human seminal plasma
2009/2/12
Parathyroid hormone-related protein (PTHrP) is a regulatory peptide that has been associated with normal fetal growth and differentiation as well as the regulation of fetal calcium. We recently d...
Immunoreactive ubiquitin in human seminal plasma
Immunoreactive ubiquitin human seminal plasma
2009/2/12
The polypeptide ubiquitin, up to now almost exclusively discovered in intracellular spaces, was measured immunologically in a total of 187 samples of human seminal plasma. The values were between 1....
Quantification of the nonenzymatic fast and slow TRAP in a postaddition assay in human seminal plasma and the antioxidant contributions of various seminal compounds
Quantification nonenzymatic fast and slow TRAP postaddition assay human seminal plasma seminal compounds
2008/12/25
Total radical-trapping antioxidant potential (TRAP) measurements of human seminal plasma (N = 25) were performed by using a post-addition assay based on trapping 2,2' Azino-bis(3-ethylbenzthiazoline...
Factors involved in the biochemical etiology of human seminal plasma hyperviscosity
biochemical etiology human seminal plasma hyperviscosity
2008/12/25
Semen rheology was studied to elucidate the biochemical basis of seminal plasma hyperviscosity. Semen proved to fit in with a power law model, by presenting a pseudoplastic behavior. Apparent vi...
Quantification of Seminal Plasma Motility Inhibitor/Semenogelin in Human Seminal Plasma
Sperm motility inhibitor seminal vesicles asthenozoospermia
2008/12/19
Semenogelin I and II (Sg I and II) are the major components of human semen coagulum. The protein is rapidly cleaved after ejaculation by the chymotrypsin-like protease prostate-specific antigen (PSA...
Enhanced Chemiluminescence Assay vs Colorimetric Assay for Measurement of the Total Antioxidant Capacity of Human Seminal Plasma
Luminometer semen spectrophotometer Trolox reactive oxygen species
2008/12/19
Although the enhanced chemiluminescence assay is commonly used to measure the nonenzymatic total antioxidant capacity (TAC) of the human seminal plasma, it is cumbersome, expensive, and time-consu...
Complexes of Gelatinases and Tissue Inhibitor of Metalloproteinases in Human Seminal Plasma
Human seminal plasma matrix metalloproteinase
2008/12/18
Previously reported data have indicated the existence of two kinds of matrix metalloproteinases (MMP-2 and MMP-9) in human seminal plasma (Shimokawa et al, 2002). Here we report the existence of com...