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Immunocytochemical localization of acrosin on both acrosomal membranes and in the acrosomal matrix of porcine spermatozoa
spermatozoa acrosin electron microscopy
2009/2/19
Immunocytochemical techniques were employed to determine, at the ultrastructural level, the location of acrosin in porcine spermatozoa. Antisera to highly purified porcine acrosin was produced in ...
Characterization of boar sperm plasma membranes by two-dimensional PAGE and isolation of specific groups of polypeptides by anion exchange chromatography and lectin affinity chromatography
plasma membranes spermatozoa twodimensional
2009/2/19
High resolution, two-dimensional PAGE was used to characterize purified plasma membranes (PM) from boar spermatozoa. In addition to the abundance of polypeptides resolved, unusual features of PM dis...
Electrophoretic and Chromatographic Properties of Boar Sperm Plasma Membranes：Antigens and Polypeptides with Affinity for Isolated Zonae Pellucidae
sperm plasma membrane zonae pellucidae
2009/2/19
Two methods were used to assess the ability of plasma membrane (PM) polypeptides to bind to isolated zonae pellucidae (ZP). Crossed immunoelectrophoresis patterns of detergent solubilized PM were co...
Cryopreservation in different concentrations of glycerol alters boar sperm and their membranes
Swine sperm motility acrosome membrane fluidity
2008/12/29
To test the hypothesis that glycerol would concomitantly affect sperm membrane structure and the function of the intact cells, boar semen (4 ejaculates from 4 boars) was cryopreserved in an egg yo...
A Putative 12-Transmembrane Domain Cotransporter Associated With Apical Membranes of the Epididymal Duct
Canine epididymis transporter TSCOT homologue apical membrane compartment
2008/12/19
The epithelial cells lining the epididymal duct play an important role in establishing and maintaining the luminal fluid microenvironment. A cDNA, canine epididymal cDNA 11 (CE11), was cloned from...