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Quantification of bovine sperm separation by a swim-up method. Relationship to sperm motility, integrity of acrosomes, sperm migration in polyacrylamide gel and fertility
Quantification bovine sperm separation swim-up method
2009/2/23
The number of bovine spermatozoa separated in a swim-up procedure was quantified using an electronic cell counter. In an initial test of the swim-up procedure, non-frozen sperm samples with differen...
Bovine sperm adenylate cyclase. Inhibition by adenosine and adenosine analogs
adenosine epididymal spermatozoa adenylate cyclase inhibition P-site
2009/2/20
Since both adenosine and cAMP affect sperm motility and metabolism, the authors have studied the mode of interaction of adenosine with the adenylate cyclase activity in either the presence or the ab...
Catalase and oviductal fluid reverse the decreased motility of bovine sperm in culture medium containing specific amino acids
Hydrogen peroxide oviduct secretions toxicity
2009/2/17
The motility and velocity of bovine spermatozoa incubated in TCM-199 are reduced in comparison with those incubated in a simpler media made for sperm, such as modified Tyrode (Sp-Talp). Moreover, ...
The impact of reactive oxygen species on bovine sperm fertilizing ability and oocyte maturation
Spermatozoa fertilization development xanthine oxidase
2009/2/17
The objective of this study was to examine the effects of reactive oxygen species (ROS) on bovine sperm function and on the developmental competence of in vitro-matured bovine oocytes. In a first se...
Effect of semen dilution on bovine sperm viability as determined by dual-DNA staining and flow cytometry
Sperm concentration cellular viability SYBR-14
2009/2/17
Living and dead spermatozoa were examined for the effects of sperm concentration level on sperm viability. Semen was collected from two different bulls on each of four collection dates. A ninth bull...
Computerized imaging and scanning electron microscope（SEM）analysis of co-cultured fresh and frozen bovine sperm
Acrosome sperm computerized imaging scanning electron microscopy oviduct
2009/2/17
Fresh and frozen-thawed bull sperm were incubated with bovine oviductal epithelial cells and segments from the oviducts to examine the usefulness of these culture systems to model sperm changes in...
Dual DNA staining assessment of bovine sperm viability using SYBR-14 and propidium iodide
Sperm fluorescence SYBR-14 flow cytometry vital staining cryopreservation
2009/2/13
A new membrane-permeant DNA stain, SYBR-14, was used in combination with propidium iodide (PI) to estimate the proportion of living sperm in bovine semen. The SYBR-14 stained living sperm while PI...
Effect of follicular or oviductal fluids on movement characteristics of bovine sperm during capacitation in vitro
Sperm motion analysis motility hyperactivation
2009/2/12
Mammalian sperm exhibit characteristic motility changes associated with capacitation. Movement characteristics of bovine sperm incubated in noncapacitating (control, medium alone), capacitating (ovidu...
Measurement of bovine sperm nuclear shape using Fourier harmonic amplitudes
bovine sperm nuclear shape
2008/12/29
An objective method for measuring bovine sperm nuclear shape was developed. Digital images of bovine sperm stained with propidium iodide were collected and Fourier functions used to describe the per...
Epididymal epithelial cells cultured in vitro prolong the motility of bovine sperm
Epididymal epithelial cells vitro motility of bovine sperm
2008/12/25
It is well known that the epididymis is an excellent environment to maintain sperm viability. Therefore, we used different sections of bovine epididymis (caput, corpus, and cauda) to develop epithel...
An immunochemical assay to detect DNA damage in bovine sperm
immunochemical assay DNA damage bovine sperm
2008/12/25
An immunochemical assay has been developed to detect oxidative damage in bovine sperm DNA. Sperm DNA contains a large amount of oxidative damage as a result of exposure to exogenous agents, but dama...
DNA Damage in Bovine Sperm Does Not Block Fertilization and Early Embryonic Development But Induces Apoptosis After the First Cleavages
Mitotic spindles blastocyst ICSI
2008/12/23
The main goal of this study was to investigate whether and at what level damage of paternal DNA influences fertilization of oocytes and early embryonic development. We hypothesized that posttesticul...
Interaction of PDC-109, the Major Secretory Protein From Bull Seminal Vesicles, With Bovine Sperm Membrane Ca2+-ATPase
Seminal vesicles bovine sperm plasma membrane Ca2+-ATPase
2008/12/22
PDC-109 is the prevalent secretory protein from bovine seminal vesicles that binds to the midpiece of sperm once they pass the ampulla of the vas deferens during emission. Thereby, the protein cha...
Implication of Calmodulin-Dependent Phosphodiesterase Type 1 During Bovine Sperm Capacitation
Vinpocetine rolipram cyclic AMP in vitro fertilization motility
2008/12/18
Phosphodiesterases (PDEs) are enzymes that degrade cyclic nucleotides. The calcium-calmodulin dependent PDE type 1 (PDE 1) and the cyclic adenosine monophosphate (cAMP)-specific PDE type 4 (PDE 4) h...