搜索结果: 1-8 共查到“23S rRNA”相关记录8条 . 查询时间(0.062 秒)
Studies of the complex of ribosomal protein L1 with its binding site in 23S rRNA: Modification -interference, mutagenesis and crosslinking
Ribosomal proteins escherichia coli ribosomes the protein spiral protein
2014/12/11
Interaction of ribosomal protein L1 and the 23S rRNA plays an important role in both the structure and biological activity of the Escherichia coli ribosome. We have minimized the binding site for prot...
Changes in the mutual orientation of tRNA and 23S rRNA at the peptidyl transferase center of the ribosome detected by crosslinking of a photoreactive transition-state analog
Amino acids dynamic interaction ribosomes peptide bonds peptidyl transferase ribosomes
2014/12/11
Dynamic interactions between the amino acid acceptor end of tRNA and the ribosome underlie the synthesis of successive peptide bonds at the peptidyl transferase center (PTC) of the 50S ribosomal subun...
Probing the Peptidyl Transferase Center of Ribosomes Containing Mutant 23S rRNA with Photoreactive tRNA
ribosome peptidyl transferase center photoreactive crosslinking tRNA 23S rRNA translation
2014/12/9
There is strong crystallographic evidence that the 23S rRNA is the only catalytic entity in the peptidyl transferase center. Various mechanisms for the catalysis of peptidyl transfer have been propose...
探索5S～23S rRNA和ospA基因序列在伯氏疏螺旋体检测中的应用, 了解浙江省鼠中感染伯氏疏螺旋体状况。方法 用PCR方法, 检测磐安县100只鼠标本中5S～23S rRNA和ospA基因特异片段。对检测到的阳性结果进行测序。结果 检测100只鼠标本, 其中3只鼠标本伯氏疏螺旋体的5S～23S rRNA基因片段为阳性, 5只鼠标本伯氏疏螺旋体的ospA基因片段为阳性, 与参考核酸序列基本一致...
2012年初，山东菏泽某羊场的羊群发病，从发病羊器官分离到2株病原细菌。对病原细菌进行鉴定，并对其与已知同种异源菌株相似性差异进行分析。从患病山羊内脏器官分离细菌，经形态特征、培养特性、生化试验、血清学试验及致病性试验进行鉴定；再通过设计通用引物扩增16S-23S rRNA ISR (intergenic spacer region)序列，将PCR产物经HinfⅠ单酶切获得3条可视条带，同时对扩增...
作者拟探讨禽源支原体对替米考星耐药的分子机制。体外诱导得到鸡毒支原体（R株、PG31株和S6株）、鸡滑液支原体、衣阿华支原体的替米考星耐药株；PCR扩增原始敏感株和诱导耐药株的23S rRNA基因V域，测序分析耐药相关碱基突变情况。结果鸡毒支原体R株、PG31株、S6株、鸡滑液支原体、衣阿华支原体分别通过9代、8代、6代、14代、9代诱导获得替米考星耐药（≥128 μg·mL-1）株；3种禽源支原...
Detection of Mutation in 23S rRNA in Isolates of Streptococcus pyogenes Resistant to Erythromycin
Streptococcus pyogenes 23S rRNA erythromycin-resistance
2009/12/17
Background: In this study, erythromycin resistance isolates from the students with aged 12-15 yr old were studied and the mutations in the 23S rRNA were identified by sequencing.Methods: Throat sample...
Identification and Comparison of the Nucleotide Sequence of 16S-23S rRNA Gene Intergenic Small SR(Spacer Region) of Lactobacillus rhamnosus ATCC 53103 with Those of L. casei, L. acidophilus and L. helveticus
16S-23S Intergenic Spacer Region Identification L. rhamnosus
2016/4/14
Reliable PCR based identification of lactobacilli has been described utilizing the sequence of 16S-23S rRNA intergenic spacer region. Those sequence comparisons showed a high degree of difference in h...