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Biofilm formation by Pseudomonas aeruginosa and disinfectant susceptibility of planktonic and biofilm cells
mode of existence disinfection fluorescence microscopy
2016/7/6
Epifluorescence microscopy (EFM) was used to study the biofilm formation of Pseudomonas aeruginosa after 6, 24, 30, 48, 54, 72, 78, and 96 h growth in a chamber slide system. For this purpose, the bio...
Molecular mechanisms of ceftazidime resistance in Pseudomonas aeruginosa isolates from canine and human infections
ampC ampR extended spectrum β-lactamases
2015/4/8
Sixty-six clinical P. aeruginosa isolates, 17 obtained from canine otitis specimens and 49 received from human patients with bloodstream infections, were collected between February 2007 and January 20...
Oxidative stress responses in Escherichia coli and Pseudomonas aeruginosa
Aerobic respiration reactive oxygen species ROS damage biological molecules cells detoxification oxidative stress transcription factors signal differential gene expression escherichia coli pseudomonas aeruginosa
2015/1/28
Aerobic respiration generates unavoidable reactive oxygen species (ROS, detrimental by-products that damage biological molecules. The accumulation of ROS beyond the cellular capacity for detoxificatio...
Structural studies of membrane-assembled PopD and PopB, the Pseudomonas aeruginosa type 3 secretion translocators
Pure sciences Biological sciences Fluorescence Membrane proteins Membranes Pore formation Type III secretion
2014/12/10
Transport of proteins across membranes is essential during many stages of pathogen infection and colonization of human cells. Many Gram-negative pathogens use a Type 3 Secretion (T3S) system to inject...
Virulence Gene Profiles of Multidrug-Resistant Pseudomonas aeruginosa Isolated From Iranian Hospital Infections
Pseudomonas aeruginosa Virulence Factors Hospital infections Iran
2015/10/15
Background: The most common hospital-acquired pathogen is Pseudomonas aeruginosa. It is a multidrug resistant bacterium causing systemic infections.
Role of a putative bacterial lipoprotein in Pseudomonas aeruginosa-mediated cytotoxicity toward airway cells
Pseudomonas aeruginosa infection Cystic Fibrosis Cytotoxicity
2015/5/22
The patients with Cystic fibrosis (CF), an inherent genetic disorder, suffer from chronic bacterial infection in the lung. In CF, modification of epithelial cells leads to alteration of the lung envir...
于2011年4-8月按月对巢湖市水源保护湖区不同水深及不同区位的铜绿微囊藻藻团粒径进行抽样调查,利用统计分析方法,归纳了巢湖水源保护湖区铜绿微囊藻藻团粒径的时空分布规律.湖区铜绿微囊藻藻团出现在4月中旬至5月中旬之间,S1与S2点位表层的藻团粒径与中、底层的均存在显著差异.粒径小于200μm的藻团在各水深的分布都比较均匀,没有明显的趋向性;粒径在200~800μm范围内的藻团更易集中在湖水表层;粒...
Studies on Extracting Microcystin-Lr from Microcystis Aeruginosa by Water Bath
Boiling Water Bath Extract Microcystin-LR
2013/3/9
Different temperatures of water bath was used to extract the intracellular microcystin-LR(MC-LR) of Microcystis aeruginosa. Researching the extraction efficiency under the suitable temperature, so tha...
选择我国蓝藻水华的优势藻类铜绿微囊藻,进行人工模拟UV-B辐射连续24h照射实验,测定铜绿微囊藻生物量、生理及超微结构指标,探讨湖泊特别是高原湖泊藻类生长对UV-B辐射增强的胁迫响应.结果表明,当辐射时间达到1h时,铜绿微囊藻便开始陆续死亡,但蛋白质含量增加,且叶绿素a浓度保持稳定;当辐射时间少于4h时,细胞内超氧化物歧化酶(SOD)始终保持较高活性,细胞膜保持基本完整,细胞内类囊体和蓝藻素颗粒数...
采用广谱性固相萃取小柱富集以10g(FW)/L的密度培养三天后的苦草(Vallisneria spiralis)种植水,不同溶剂洗脱得到的各组分对铜绿微囊藻(Microcystis aeruginosa)的生长表现出不同程度的抑制,其中甲醇洗脱组分抑藻活性最强.去除该组分中的酚酸后,其抑藻活性下降了22.8%,表明酚酸参与到苦草的化感抑藻作用中.甲醇组分通过液液萃取进一步分离,得到的乙酸乙酯组分进...
进入湖泊中不同氮源氮稳定同位素值( δ 15 N )的差异和生物对氮稳定同位素的记忆作用,可以反映流域人类活动输入的污染物对生态系统的影响程度 . 本文调查了太湖 4 个湖湾(梅梁湾、贡湖湾、竺山湾和东太湖)中铜锈环棱螺( Bellamya aeruginosa )的 δ15 N 值,结果表明环棱螺 δ15 N 值的变幅为 6.9‰~18.1‰ ,平均值为 11.2‰ ,不同湖湾中环棱螺 δ 15...
对三峡水库一级支流神农溪 2008 年夏季蓝藻水华进行调查, 结果表明神农溪蓝藻水华优势种是铜绿微囊藻 ( Microcystis aeruginosa ) ,暴发水域面积约 0.2km 2 ,持续时间约 50d. 本次水华暴发与水体总磷浓度呈正相关,温度、降雨、光照和营养盐等对水华暴发具有重要影响 . 研究发现尽管神龙溪水域总体处于中富营养状态,但局部水域由于水流缓慢、水体滞留时间长、营养稀释扩...
为研究生长环境中微生物对铜绿微囊藻碳代谢的影响,本文分析太湖典型微囊藻水华样品附生菌中产碳酸酐酶(CA)细菌的比例,结果显示CA菌占11.6%;从微囊藻群体中分离获得了一株高胞外CA附生菌P201,通过ITS基因鉴定,该菌为一株荧光假单胞菌(Pseudomonas fluorescence).并研究了该菌在不同浓度HCO-3条件下对铜绿微囊藻生长的影响,结果表明无论是高HCO-3浓度还是低HCO-...
在实验室光照黑暗交替培养下,用半胱氨酸下调黑暗起始时的氧化还原电位( ORP ),研究低电位对铜绿微囊藻( Microcystis aeruginosa FACHB 469 )生长的影响,并探索低电位胁迫消除后铜绿微囊藻生长的恢复潜力 . 结果表明,在 -50~-100mV 范围内水体 ORP 越低,其对铜绿微囊藻生长的抑制作用越强 . 低 ORP 可抑制铜绿微囊藻对磷的吸收,且这种抑制程度与电位...
光照强度对铜绿微囊藻(Microcystis aeruginosa)和斜生栅藻(Scenedesmus obliqnus)生长及吸收特性的影响
铜绿微囊藻 斜生栅藻 吸收系数 比吸收系数 光照强度
2012/9/11
在不同的光照强度下,测定了铜绿微囊藻、斜生栅藻在不同生长期的藻细胞密度、粒径、叶绿素a浓度、浮游植物吸收系数以及比吸收系数.单因素方差分析表明,在整个培养周期中,光照强度对铜绿微囊藻及斜生栅藻的藻细胞密度、叶绿素a浓度以及440、675nm处吸收系数均有着显著的影响.两种藻在光照强度为50μmol/(m2·s)条件下,藻细胞密度、叶绿素a浓度及吸收系数值相对最大.相关性分析表明:藻类特征波段440...