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This study was conducted to examine the effects of different soy-oil and soy-lecithin levels on growth performance, nutrient utilization and serum parameters in broiler chickens. Two hundred and forty...
In an attempt to speed up the process of screening of transgenic cotton (G. hirsutum L.) plants, a visual and rapid loop-mediated isothermal amplification (LAMP) assay was adopted. Genomic DNA was ext...
Opportunistic fungal infections including candidiasis have increased dramatically in recent years. Most medically important fungi are Candida species. Rapid identification od Candida isolates to the s...
Background: In the present study, a PCR-RFLP based molecular technique was designed to rapid identification of der­matophytes in clinical specimens. Skin scrapings obtained from human cases suspec...
Emergent antimicrobial resistance in bacteria is one of the world's most pressing health problems, and a major contributor to patient morbidity and mortality. Both the generation of new resistant stra...
To develop a new method for identification of Listeria monocytogenes genetically similar to clinical isolates, single-nucleotide polymorphism (SNP) typing and multi-locus sequence typing (MLST) of 126...
Mutans group streptococci play an important role in the formation of dental caries, while mitis group streptococci are considered to be associated with subacute bacterial endocarditis. Both mitis and ...
摘要 根据已报道的序列,把苦瓜(Momordica charantia) MAP30基因成功克隆到原核表达载体pET28a(+)中,并对PCR快速检测阳性克隆进行了研究。结果表明,直接用菌落、菌液、酚氯仿处理过的菌液,以及提取的质粒进行PCR都可以成功地筛选阳性菌落。其中,酚氯仿处理过的菌液PCR与质粒PCR的结果最接近,而且比质粒PCR简单,因此可作为方便可靠的阳性克隆筛选的新方法。Abstr...

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