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Purification and Characterization of Glutamate Decarboxylase from Aspergillus oryzae
glutamate decarboxylase Aspergillus oryzae purification
2009/6/17
We purified glutamate decarboxylase (GAD) [EC4.1.1.15] from Aspergillus oryzae and characterized its biochemical and kinetic properties. GAD was purified by ammonium sulfate at 30–70% saturation and c...
Effect of helium and heliox on glutamate decarboxylase activity
helium heliox air glutamate decarboxylase
2009/3/30
The activity of glutamate decarboxylase (GAD) in pure helium at 6.8 MPa was significantly enhanced when compared to GAD activity in air at 0.1 MPa (906 vs. 602 nmol.h-1.mg-1 protein, respectively). No...