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Rapid and visual detection of 2019 novel coronavirus (SARS-CoV-2) by a reverse transcription loop-mediated isothermal amplification assay
SARS-CoV-2 RT-LAMP COVID-19 Detection Visual
2020/6/12
To evaluate a reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay for detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), and compare it with RT-PCR.
Detection of Klebsiella pneumonia in respiratory tract samples by the loop-mediated isothermal amplification method
环介导等温扩增技术 呼吸道感染 肺炎克雷伯菌 细菌培养法 DNA测序技术
2021/3/29
建立环介导等温扩增技术(loop-mediated isothermal amplification,LAMP)检测呼吸道感染患者痰液中肺炎克雷伯菌(Klebsiella pneumoniae,Kpn)的方法并进行初步应用。 方法:基于Kpn phoE基因设计4条特异性引物,对反应条件中的Mg2+和甜菜碱浓度进行优化,建立检测Kpn LAMP方法并进行特异性和灵敏度试验;收集308例呼吸道感染患...
Rapid Identification of Transgenic Cotton (Gossypium hirsutum L.) Plants by Loop-mediated Isothermal Amplification
Bt chitinase CryIA(b) detection loop-mediated isothermal amplification transgenic cotton
2014/2/25
In an attempt to speed up the process of screening of transgenic cotton (G. hirsutum L.) plants, a visual and rapid loop-mediated isothermal amplification (LAMP) assay was adopted. Genomic DNA was ext...
Influence of Bovine Serum Individual Difference in Viral Detection Using Loop-mediated Isothermal Amplification and Metal-coated Hollow Fiber Membrane
blood products serum individual difference viral detection metal-coated hollow fiber membrane
2009/6/9
In this study, we used two kinds of simulated serum samples that used the bovine serums of different lot numbers and reviewed stability for slight differences in blood components in terms of the desig...