搜索结果: 1-15 共查到“myostatin”相关记录38条 . 查询时间(0.123 秒)
Knockout of Myostatin by Zinc-finger Nuclease in Sheep Fibroblasts and Embryos
Myostatin Zinc-finger Nucleases Knockout, Sheep
2016/11/10
Myostatin (MSTN) can negatively regulate the growth and development of skeletal muscle, and natural mutations can cause “double-muscling” trait in animals. In order to block the inhibiting effect of M...
Targeted Editing of Myostatin Gene in Sheep by Transcription Activator-like Effector Nucleases
Myostatin Targeted Gene Editing Sheep Primary Fibroblasts
2016/5/19
Myostatin (MSTN) is a secreted growth factor expressed in skeletal muscle and adipose tissue that negatively regulates skeletal muscle mass. Gene knockout of MSTN can result in increasing muscle mass ...
L-leucine, beta-hydroxy-beta-methylbutyric acid (HMB) and creatine monohydrate prevent myostatin-induced Akirin-1/Mighty mRNA down-regulation and myotube atrophy
Myostatin GDF8 Akirin-1
2015/7/29
After four days of differentiation, myotubes were treated with MSTN (10 ng/ml) for two additional days and four treatment groups were studied: 1) 3x per day 10 mM Leu, 2) 3x per day 10 mM HMB, 3) 3x p...
Expression profiles of myostatin and calpastatin genes and analysis of shear force and intramuscular fat content of yak longissimus muscle
Bos grunniens meat trait muscle growth hypoxia intramuscular fat
2015/6/9
The main objective of this study was to reveal the expression profiles of two negative regulators, myostatin (MSTN) and calpastatin (CAST)genes, of skeletal muscle growth in highland yaks (Bos grunnie...
Fundamental study of detection of muscle hypertrophy-oriented gene doping by myostatin knock down using RNA interference
In vitro and in vivo transformation C2C12 cultured cell
2015/5/11
To investigate the feasibility of developing a method for detection of gene doping in power-athletes, we devised an experimental model system.
A single nucleotide polymorphism in exon 3 of the myostatin gene in different breeds of domestic pigeon (Columba livia var. domestica) 32
myostatin polymorphism synonymous mutation muscle growth
2015/4/17
Myostatin is considered to be one of the most powerful negative regulators of muscle growth. The lack of a functional myostatin or its mutation can result in uncommon musculature like “double-muscling...
Estradiol In Females May Negate Skeletal Muscle Myostatin Mrna Expression And Serum Myostatin Propeptide Levels After Eccentric Muscle Contractions
Estrogen muscle damage eccentric contractions myostatin
2015/3/23
Eccentric contractions produce a significant degree of inflammation and muscle injury that may increase the expression of myostatin. Due to its anti- oxidant and anti-flammatory effects, circulating 1...
EFFECTS OF CONCENTRIC AND ECCENTRIC MUSCLE ACTIONS ON SERUM MYOSTATIN AND FOLLISTATIN-LIKE RELATED GENE LEVELS
Muscle injury cytokine muscle proteolysis resistance exercise
2015/3/13
he present study determined the effects of concentric and eccentric muscle actions on the contents of
serum myostatin and follistatin-like related gene (FLRG). Eight untrained males performed one ex...
旨在探究绵羊胚胎中后期骨骼肌BTG2/3（B cell translocation gene 2/3）的动态表达情况，探索肌肉生长抑制素(Myostatin，MSTN)基因对其表达的影响。以特克赛尔（Texel）和乌珠穆沁（Ujimqin)绵羊85、100、120和135 d 4个不同发育阶段的胎儿背最长肌为研究对象，利用Real-time PCR技术分别检测MSTN及BTG2/3的表达差异；选取...
为阐明Myostatin在肌原纤维损伤中的作用，并探讨提高畜禽肉产（质）量或控制人类肌肉萎缩的方法，本试验以小鼠为模式动物，采用高剂量地塞米松构建了严重应激模型，研究生理剂量胰岛素对地塞米松致肌原纤维损伤的影响及其与Myostatin基因表达的关系，以及Myostatin基因免疫对地塞米松致肌原纤维损伤的干预作用。结果表明：地塞米松诱导Myostatin基因表达上调及严重的肌原纤维损伤和线粒体肿胀...
试验克隆了崂山奶山羊Myostatin基因序列,构建了其真核表达载体,并验证了其在成纤维细胞中的表达。本研究通过从崂山奶山羊肌肉组织中提取RNA,反转录后采用巢式PCR方法扩增出Myostatin基因序列,构建真核表达载体,通过转染成纤维细胞,采用RT-PCR方法验证其表达。结果表明,克隆出崂山奶山羊Myostatin基因的全长cDNA序列,大小为1128 bp,GenBank登录号:GU3773...
Myostatin基因沉默绵羊成纤维细胞系的建立
肌肉生长抑制素 RNA干扰 慢病毒 绵羊成纤维细胞
2013/12/3
旨在获得肌肉生长抑制素（Myostatin，MSTN）基因沉默的成纤维细胞系，为进一步探索Myostatin的调控机理，获得 Myostatin的基因沉默的转基因羊奠定基础。针对小尾寒羊Myostatin的基因序列，设计4条siRNA干扰序列，构建pSilencer干扰载体，转染成纤维细胞，采用RTPCR进行筛选；构建慢病毒干扰载体，包装病毒，感染成纤维细胞，采用流式细胞技术分选阳性细胞，阳性细...
Effect of Myostatin (MSTN) g+6223G>A on Production and Carcass Traits in New Zealand Romney Sheep
Myostatin MSTN GDF8 g+6223G>A Sheep Carcass Trait
2016/5/5
Myostatin, which is also known as growth and differentiation factor 8 (GDF8), has been reported to act as a negative regulator of skeletal muscle development. Variation in the myostatin gene (MSTN) ha...
大黄鱼Myostatin-1基因启动子克隆及生物信息学分析
大黄鱼 Myostatin-1启动子 克隆 生物信息学分析
2015/5/29
Myostatin基因属于TGF β超家族,是动物骨骼肌生长发育的负调控因子。在已克隆大黄鱼Myostatin-1基因编码序列的基础上，采用基因组步移的方法，首次克隆到约1k bp的大黄鱼Myostatin-1启动子序列。该启动子序列与已报道的金鲷和大口黑鲈相应序列的相似度分别为90%和75%。该序列具有1个TATA box、1个CAAT box、5个E-box位点，此外还具有MyoD，USF，M...