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高通量测序分析Nisin对腹泻小鼠肠道菌群的影响
Nisin 致病性大肠杆菌 高通量测序
2019/1/16
采用高通量测序研究乳酸链球菌素(Nisin)对腹泻小鼠肠道菌群结构的影响。通过腹腔灌注E.coli O1建立腹泻病小鼠模型,通过动物体内保护率试验筛选出最佳药物剂量,将小鼠随机分为空白对照组、阴性对照组、氨苄青霉素、盐酸环丙沙星组和Nisin组。连续灌胃15 d,收集盲肠粪便,采用高通量测序对5组样品进行分析。结果表明:1)E.coli O1可使腹泻小鼠十二指肠绒毛断裂;Nisin可增加淋巴细胞百...
In vitro antagonistic effect of nisin on faecal enterococci and staphylococci
staphylococci nisin inhibition
2015/1/20
Enterococci and staphylococci, isolates from faecal samples of 46 different animals such as deer, chamois, European bison, zebra, camel, antelope, gazelle, horse, and piglets were treated by nisin (co...
用乳酸、乳酸钠与Nisin复合保鲜剂对冷却羊肉进行处理,自封袋包装后在4±1℃的环境中贮藏,测其在贮藏期间的菌落总数、挥发性盐基氮、p H和感官评分,用权重对不同配比的保鲜液对冷却羊肉的保鲜效果进行综合评估,并在此基础上进行优化。结果表明:乳酸浓度0.15%、乳酸钠浓度0.3%、乳酸链球菌素浓度为0.05%时保鲜效果最佳,该处理下的样品在贮藏20 d时各项品质指标仍能保持在鲜肉范围内。
乳酸乳球菌nisin抗性基因的克隆及作为筛选标记的研究
乳酸乳球菌 nisin抗性基因(nsr) 食品级筛选标记
2009/11/24
在添加500U/mL nisin和溴甲酚紫的GM17选择性培养基上,分离到5株nisin抗性菌株。根据形态观察,生理生化特性和16S rDNA基因序列比对被鉴定为乳酸乳球菌。根据已报道nisin抗性基因设计引物,分别以这5株菌的染色体和质粒DNA为模板进行PCR扩增,结果从1株抗性菌株的染色体上得到目的基因产物。通过序列测定和同源性比对,证明为nisin抗性基因(nsr)。将nsr基因克隆到乳酸...
Span-20和Tween-80对发酵液中Nisin泡沫分离的影响
泡沫分离 非离子表面活性剂 乳链菌肽
2009/11/13
含0.5 g/L Tween-80发酵液;Tween-80的加入破坏了气泡表面的网状结构,因而泡沫层持气量较高;连续通气时,2种表面活性剂都会引起泡沫层高度降低,但Tween-80会增加泡沫层的稳定性. 当Tween-80浓度达0.5 g/L时,Nisin的收率大幅度提高,原因是分离过程中Nisin失活率减少.
乳链菌肽发酵玉米浆—糖蜜培养基的优化制备(Optimization Corn Steep Liquor—Molasses Fermentation Medium for Nisin)
乳链菌肽 玉米浆 糖蜜 Box-Behnken设计
2009/8/11
采用单因素试验方法对影响乳链菌肽效价的培养基组分进行了考察。结果表明,影响乳链菌肽效价的主要因素为糖蜜、玉米浆、K2HPO4、MgSO4。在此基础上,采用Box-Behnken设计及响应面分析法确定主要影响因子的最佳质量浓度。结果表明,当玉米浆质量浓度为233g/L,糖蜜质量浓度为27g/L,K2HPO4质量浓度为5.0g/L,MgSO4质量浓度为0.1g/L时,乳链菌肽效价为2491.8IU/m...
Nisin与山楂提取物复配的抑菌性能研究
Nisin 山楂 抑菌性
2009/7/13
[ 目的] 为开发新型天然防腐剂提供依据。[ 方法] 以山楂为材料, 枯草芽孢杆菌、酿酒酵母和大肠杆菌为供试菌种, 采用传统水煎法提取山楂中的有效成分, 并向10 、20 、30 、40 、50 、60 、70 、80 、90 、100 μl 提取物中分别加入10 mg Nisin 制成复配物, 测定复配物对3 种供试菌的抑菌效果。[ 结果] Nisin 的效价为994 .5 IU/ mg , ...
将Nisin、NaL 单独及联合应用于真空包装冷却猪肉中, 通过测定菌落总数的变化来分析Nisin、NaL 在贮藏过程中的交互效应。结
果表明, 单独使用Nisin、NaL 均可显著抑制菌落总数的产生, 且浓度越高抑菌效果越好; 贮藏前期Nisin 抑菌效果较好, 贮藏后期3 %NaL抑菌效果最好;Nisin、NaL 联合应用比单独使用抑菌效果要好, 而且两者在整个贮藏期间均显示显著的交互效应。...
Searching for Genes of Lactococcus lactis subsp. lactis Encoding the Bacteriocin Nisin using DNA/DNA Hybridisation
Lactococcus lactis subsp .lactis screening nisin gene PCR probe DNA/DNA hybridisation
2014/3/10
n this work, PCR primers P8 and P9 were used for amplification of a 320 bp long PCR product specific to the nisin gene. The PCR product was labelled with digoxigenine during amplification and used as ...
Identification and characterisation of antimicrobial activity of nisin a produced by Lactococcus lactis subsp. lactis LL27
nisin A Lactococcus lactis sublethal injury heating freezing
2014/3/10
In this study, bacteriocin producing lactococcal strains were isolated from Turkish raw milk samples. Among these isolates, LL27 had the highest inhibition activity against Gram-positive indicator str...
该项目应用色谱-质谱联用仪和生物新技术等解析偶联物的分子构像,确定偶联物理化及生物学性质,同时采用不同的偶联合成和分离纯化技术进行偶联物的优化筛选,并通过体内动物实验对偶联物抗幽门螺杆菌感染的药效进行系统评价,可望为防治幽门螺杆菌感染性疾病提供有效的、有自主知识产权的新型抗菌剂。
Increased Nisin in Cheddar-Type Cheese Prepared with pH Control of the Bulk Starter Culture System
nisin lactic acid bacteria cheese
2008/3/18
Five strains of Lactococcus lactis ssp. lactis and one transconjugant strain of Lactococcus lactis ssp. cremoris, previously shown to be nisin producers, were evaluated for nisin production during gro...
Shelf-Life of Pasteurized Process Cheese Spreads Made from Cheddar Cheese Manufactured with a Nisin-Producing Starter Culture
cheese spreads nisin spoilage shelf-life
2008/3/18
Cheddar cheese made with a nisin-producing starter culture and Cheddar cheese made with a commercially available starter culture were used to manufacture pasteurized process cheese spreads at low and ...