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Sex Determination of Cattle Meat by Polymerase Chain Reaction Amplification of the DEAD Box Protein (DDX3X/DDX3Y) Gene
Cattle Meat Sex Determination Meat Quality DEAD Box Protein Gene
2016/5/10
Determination of sex origin of cattle meat by fast and reliable molecular methods is an important measure to ensure correct allocation of export refunds particularly in European countries and also fem...
Nonnormal amplification in random balanced neuronal networks
Nonnormal amplification random balanced neuronal networks Neurons and Cognition
2012/4/28
In dynamical models of cortical networks, the recurrent connectivity can amplify the input given to the network in two distinct ways. One is induced by the presence of near-critical eigenvalues in the...
Rapid Identification of Transgenic Cotton (Gossypium hirsutum L.) Plants by Loop-mediated Isothermal Amplification
Bt chitinase CryIA(b) detection loop-mediated isothermal amplification transgenic cotton
2014/2/25
In an attempt to speed up the process of screening of transgenic cotton (G. hirsutum L.) plants, a visual and rapid loop-mediated isothermal amplification (LAMP) assay was adopted. Genomic DNA was ext...
General-use polymerase chain reaction primers for amplification and direct sequencing of enolase, a single-copy nuclear gene, from different animal phyla
enolase Metazoa, scnDNA
2015/12/11
The dearth of general-use (i.e. quasi-‘universal’) primer
sets for the amplification of single-copy nuclear DNA
(scnDNA) loci, combined with the availability and ease of
use of mitochondrial (mtDNA...
Amplification of Porcine SRY Gene for Sex Determination
SRY Gene Sex Determination Sperm Sorting Duplex PCR
2016/4/29
The separation of X and Y chromosome-bearing sperm is of use in many aspects of livestock maintenance. In this study, we sought to determine the difference in DNA content between X- and Y-bearing sper...
cDNA末端快速扩增（RACE）技术是一种快速获得cDNA的3′和5＇端的方法。本文从RACE的原理出发，指出其技术本身存在的优缺点，阐述了RACE操作中须注意和不容忽视的技术要点，并对前人对RACE技术所做的改进加以总结，最后对RACE技术的应用前景给予了展望。Abstract: Rapid amplification of cDNA end (RACE) technique is a meth...