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Genetic engineering and personalized cell therapies could transform health care. In recent years, stem cells and gene-editing tools like CRISPR have been making headlines for the possibilities they of...
Modern computational tools open new era of fossil pollen research（图）
Modern computational tools new era fossil pollen
2020/11/6
One of the best sources of information on the evolution of terrestrial ecosystems and plant diversity over millions of years is fossil pollen. For palynologists -- the scientists who study ancient pol...
International Conference on Forest Research in the Big Data Era
International Conference Forest Research in the Big Data Era
2017/8/31
It is our great pleasure to announce the Inaugural GFBI Conference & Joint GFBI-FECS Symposium, to be held in Beijing, China, on September 6-9, 2017.Forests are critical in the fight against biodivers...
This imaging technology provides unprecedented 3-D views of an intact brain's neural structure and its vast internal connections
The connections between neurons in the brain are involved in everythin...
The University of Queensland will lead a national collaboration at the forefront of linking science to ecosystem management thanks to the establishment of a new research network.
The Terrestrial Ec...
To construct the bait vector with different domains of human Era in yeast two-hybrid system, then assay whether its expression product can affect the growth of yeast cells and activate the reporter ge...
大肠杆菌中高表达重组Era可溶性蛋白的纯化及其生化特性
Era蛋白 基因表达 蛋白质纯化 G蛋白
2007/12/20
摘要 质粒pCE31含有在P_L起动子控制下的重组era基因,在大肠杆菌中、42℃诱导高表达出Era蛋白,经溶菌酶处理裂菌、沉淀离心洗涤后所得的纯Era是不溶的,生物活性也不高。改在40℃诱导2h,在Era底物GDP的存在下裂菌,60％以上的Era处于可溶状态。用蛋白酶抑制剂防止Era蛋白降解,经盐析、Q-SepharoseFF柱层析分离,获得可溶性纯Era蛋白。该蛋白能特异地与鸟苷酸结合、并具有...
大肠杆菌中rnc与era基因的共表达
rnc基因 erd基因 基因表达
2007/12/20
摘要 rnc,era基因是大肠杆菌两个相邻的基因。用S_1核酸酶图谱法测得转录从rnc基因伸延到era基因。当rnc基因突变使所产生的RNaseⅢ丧失活性时,大肠杆菌中RNaseⅢ和Era蛋白的合成速度同对上升,且上升幅度相同,合成量相等。将此二基因分别或一起克隆入质粒并置于P_L起动子下游时,rnc能表达产生RNaseⅢ;rnc-era能表达产生RNaseⅢ和Era两种蛋白质,单独era则虽能转...
Fibrobacter succinogenes, a Dominant Fibrolytic Ruminal Bacterium: Transition to the Post Genomic Era
Cellulose Cell Walls Fibrobacter succinogenes Cellulase Xylanase Fibrobacter intestinalis
2016/4/25
Fibrobacter succinogenes, a Gram-negative, anaerobic ruminal bacterium is a major fibre digesting species in the rumen. It intensively degrades plant cell walls by an erosion type of mechanism, burrow...