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Vibrational probes can provide a direct readout of the local electrostatic field in complex molecular environments, such as protein binding sites and enzyme active sites. This information provides an ...
Three different nitrile-containing amino acids, p-cyanophenylalanine, m-cyanophenylalanine, and S-cyanohomocysteine, have been introduced near the active site of the semisynthetic enzyme ribonuclease ...
The vibrational Stark effect (VSE) has proven to be an effective method for the study of electric fields in proteins via the use of infrared probes. To explore the use of VSE in nucleic acids, we inve...
Infrared spectroscopy is widely used to probe local environments and dynamics in proteins. The introduction of a unique vibration at a specific site of a protein or more complex assembly offers many a...
Nitric oxide (NO) binds to the myoglobin (Mb) cavity mutant, H93G, forming either a 5- or 6-coordinate Fe--NO heme complex. The H93G mutation replaces the proximal histidine of Mb with glycine, allowi...
Cholesterol is an essential component of mammalian cell membranes and it is important to regulate the structure and function of lipid bilayers. Changes in cholesterol levels are involved in many physi...
摘要本文简述了植物特异性DNA探针的种类,介绍了特异性探针的制备方法,主要是特异性DNA序列的分离与筛选,总结了植物特异性DNA探针在种质鉴定、外源染色体识别、核型分析和基因组研究等方面的应用,提出了存在的问题与应用前景。 Abstract:In this paper,the types of specific DNA probes from plant were briefly describ...
专著信息 书名 Accurate identification of closely related Dendrobium species with multiple species-specific gDNA probes 语种 英文 撰写或编译 作者 Tong Xiang Li,JinKe Wang,ZuHong Lu. 第一作者单位 出版社 Journal of Biochemical an...
专著信息 书名 DNA microarrays with unimolecular hairpin double-stranded DNA probes: fabrication and exploration of sequence-specific DNA/protein interactions. 语种 英文 撰写或编译 作者 Jinke Wang,Yunfei Bai,Tongxiang ...

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