搜索结果: 1-15 共查到“生物化学 dynamics”相关记录18条 . 查询时间(0.117 秒)
Site-Specific Measurement of Water Dynamics in the Substrate Pocket of Ketosteroid Isomerase Using Time-Resolved Vibrational Spectroscopy
IL-33 ST2 MWCNTs pulmonary fibrosis nanotoxicology
2016/5/25
Little is known about the reorganization capacity of water molecules at the active sites of enzymes and how this couples to the catalytic reaction. Here, we study the dynamics of water molecules at th...
Ribonuclease S Dynamics Measured Using a Nitrile Label with 2D IR Vibrational Echo Spectroscopy
Anthraquinonic dyes Boron-doped diamond anode Metal catalysts Solar photoelectro-Fenton Wastewater treatment
2016/5/25
A nitrile-labeled amino acid, p-cyanophenylalanine, is introduced near the active site of the semisynthetic enzyme ribonuclease S to serve as a probe of protein dynamics and fluctuations. Ribonuclease...
Ultrafast Excited-State Dynamics in the Green Fluorescent Protein Variant S65T/H148D.2.Unusual Photophysical Properties
Arabidopsis thaliana homogentisate phytyltransferase (HPT/VTE2) Lactuca sativa tocopherol tocopherol cyclase (TC/VTE1) vitamin e
2016/5/25
In the preceding accompanying paper [Shu, X., et al. (2007) Biochemistry 46, 12005-12013], the 1.5 A resolution crystal structure of green fluorescent protein (GFP) variant S65T/H148D is presented, an...
Ultrafast Excited-State Dynamics in the Green Fluorescent Protein Variant S65T/H148D.1.Mutagenesis and Structural Studies
Arabidopsis thaliana homogentisate phytyltransferase (HPT/VTE2) Lactuca sativa tocopherol tocopherol cyclase (TC/VTE1) vitamin e.
2016/5/24
Wild type green fluorescent protein (wt-GFP) and the variant S65T/H148D each exhibit two absorption bands, A and B, which are associated with the protonated and deprotonated chromophores, respectively...
Diffusive Dynamics of Vesicles Tethered to a Fluid Supported Bilayer by Single-Particle Tracking
Calcium Macromolecular Substances 4-Chloro-7-nitrobenzofurazan DNA Lipid Bilayers Phosphatidylcholines Coated Materials, Biocompatible Nucleic Acid Hybridization Fluorescence Recovery After Photobleaching Diffusion
2016/5/24
We recently introduced a method to tether intact phospholipid vesicles onto a fluid supported lipid bilayer using DNA hybridization (Yoshina-Ishii, C.; Miller, G. P.; Kraft, M. L; Kool, E. T.; Boxer, ...
Green Fluorescent Protein Variants as Ratiometric Dual Emission pH Sensors.2.Excited-State Dynamics
Animals Protons Deuterium Luminescent Proteins Green Fluorescent Proteins Spectrometry, Fluorescence Biosensing Techniques Kinetics Genetic Variation Hydrogen-Ion Concentration
2016/5/24
In the preceding paper [Hanson, G. T., McAnaney, T. B., Park, E. S., Rendell, M. E. P., Yarbrough, D. K., Chu, S., Xi, L., Boxer, S. G., Montrose, M. H., and Remington, S. J. (2002) Biochemistry 41, 1...
Dynamics of Myoglobin-CO with the Proximal Histidine Removed:Vibrational Echo Experiments
Myoglobin-CO Proximal Histidine Removed Vibrational Echo Experiments
2016/5/23
Picosecond infrared vibrational echo measurements from 60 to 300 K on CO bound to the active site of a mutant myoglobin, H93G(N-MeIm), are presented and compared to measurements on native myoglobin an...
Ultra-fast Excited State Dynamics in Green Fluorescent Protein:Multiple States and Proton Transfer
Protons Luminescent Proteins Green Fluorescent Proteins Recombinant Proteins Spectrometry, Fluorescence Temperature Amino Acid Sequence Protein Conformation Structure-Activity Relationship Oxidation-Reduction
2016/5/23
The green fluorescent protein (GFP) of the jellyfish Aequorea Victoria has attracted widespread interest since the discovery that its chromophore is generated by the autocatalytic, posttranslational c...
Vibrational Dynamics of Carbon Monoxide at the Active Sites of Mutant Heme Proteins
Vibrational Dynamics Carbon Monoxide Active Sites Mutant Heme Proteins
2016/5/23
Picosecond mid-IR pump−probe measurements of vibrational relaxation (VR) of CO bound to the active sites of wild-type and mutant myoglobins (Mb) reveal that an approximately linear relationship ...
Anatomy and Dynamics of a Ligand-Binding Pathway in Myoglobin:The Roles of Residues 45,60,64 and 68
Humans Carbon Monoxide Myoglobin Recombinant Proteins Ligands Spectrum Analysis Protein Binding Kinetics Mutation Thermodynamics
2016/5/23
In order for diatomic ligands to enter and exit myoglobin, there must be substantial displacements of amino acid side chains from their positions in the static X-ray structure. One pathway, involving ...
Dynamics of Protein Relaxation in Site-Specific Mutants of Human Myoglobin
Humans Cysteine Heme Alanine Myoglobin Recombinant Proteins Spectrophotometry Mutagenesis,Site-Directed Amino Acid Sequence Protein Conformation
2016/5/23
We have recently reported spectroscopic evidence for structural relaxation of myoglobin (Mb) following photodissociation of MbCO [Lambright, D. G., Balasubramanian, S., & Boxer, S. G. (1991) Chem. Phy...
Characterization of the Overall and Internal Dynamics of Short Oligonucleotides by Depolarized Dynamic Light Scattering and NMR Relaxation Measurements
Overall and Internal Dynamics Short Oligonucleotides Depolarized Dynamic Light Scattering NMR Relaxation
2016/5/23
The dynamics of three synthetic oligonucleotides d(CG)4, d(CG)6, and d(CGCGTTGTTCGCG) of different length and shape were studied in solution by depolarized dynamic light scattering (DDLS) and time-res...
Ligand and Proton Exchange Dynamics in Recombinant Human Myoglobin Mutants
Mb myoglobin n.m.r. nuclear magnetic resonance fwhm full width at half maximum TSP sodium 3-trimethyl silyl propionate DSS 4,4-dimethyl-4-sila pentane-1-sulfonate
2016/5/20
Site-specific mutants of human myoglobin have been prepared in which lysine 45 is replaced by arginine (K45R) and aspartate 60 by glutamate (D60E), in order to examine the influence of these residues ...
Receptor clusters and dynamics in kinase regulation in bacterial chemotaxis
Signal transduction bacterial chemotaxis systems membrane membrane protein cytoplasmic form complex signaling proteins
2014/12/11
Signal transduction in bacterial chemotaxis system is mediated by a family of transmembrane proteins that are clustered in the membrane and form noncovalent complexes with cytoplasmic signaling protei...
Microtubule dynamics and behavior during the assembly and disassembly of mammalian mitotic spindles
Mitosis cytoskeleton element movement protein cells genome microtubules biological polymer
2014/12/11
The mitotic apparatus is composed of a complex, seemingly chaotic, but wonderfully organized compilation of cytoskeletal elements and motor proteins. Its task is to assure that each daughter cell rece...