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Effect of Two Doses of Different Zinc Sources (Inorganic vs. Chelated form) on the Epithelial Proliferative Activity and the Apoptotic Index of Intestinal Mucosa of Early-weaned Pigs Orally Challenged with E. coli K88
Weaning Pig Zinc Oxide Small Intestine Apoptosis Mitosis
2016/5/4
The effect of two doses of different sources of zinc, inorganic (zinc oxide) or chelated (zinc glutamate chelate), on morphology and turn-over of the small intestine was assessed in early-weaned pigs ...
Anti Microbial Resistance Profile of E. coli isolates From Tropical Free Range Chickens
E. coli Anti-microbial resistance Local fowl Turkey Roosters Cockerels Nigeria
2015/7/29
Normal intestinal flora of humans and animals constitute enormous reservoir of resistance genes for potentially pathogenic bacteria and may serve as major indictors of selection pressure exerted by an...
Sensitivity of Escherichia coli to Seaweed (Ascophyllum nodosum) Phlorotannins and Terrestrial Tannins
Ascophyllum nodosum E. coli O157:H7 Phlorotannin Pure Culture Growth Cattle
2016/4/29
Pure culture experiments were conducted to assess the bacteriostatic and bactericidal effects of phlorotannins (PT) isolated from Ascophyllum nodosum (brown seaweed) on Escherichia coli O157:H7. In Ex...
Effects of Achyranthes Bidentata Polysaccharide on Growth Performance, Immunological, Adrenal, and Somatotropic Responses of Weaned Pigs Challenged with Escherichia coli Lipopolysaccharide
Achyranthes Bidentata Polysaccharide Lipopolysaccharide Inflammatory Response Weaned Pigs
2016/4/28
A study was conducted with 48 weaned barrows (283 d, 8.450.14 kg) to determine the effect of Achyranthes bidentata polysaccharide (ABPS) supplementation on pig performance, immunologic...
Identification and Screening of Gene(s) Related to Susceptibility to Enterotoxigenic Escherichia coli F4ab/ac in Piglets
ETEC F4ab/ac Susceptibility Receptor Muc4 Ep-CAM Piglets
2016/4/27
In 2004, J첩rgensen and coworkers proposed the MUC4 gene as a candidate gene of enterotoxigenic Escherichia coli (ETEC) F4ab/ac receptor in piglets and a mutation of G??C in intron 7 of MUC4 was...
Escherichia coli gal K和gpt基因注人小鼠受精卵的研究
显微注射 杂交 凝胶电泳
2008/1/21
摘要含有E. coli gaIK和gpt基因的重组DNA pPB22用显微注射法导入昆明白小鼠受精
卵，经发育，最后得到21只成年小鼠。其中1只小鼠的肝脏显示出E. coli gpt酶活性，但未检测出E. coli galK酶活性。印迹分子杂交表明，外源的pPB 22重组DNA已整合到2只小鼠的染色体上，其中包括那只肝脏中显示E. cola gpt酶活性的小鼠。
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小鼠canstatin及其N端片段在大肠杆菌BL21中的表达Expression of Mouse canstatin and its N-domain in E.coli BL21
canstatin 血管生成抑制素 RT-PCR cDNA克隆
2008/1/20
摘要 以小鼠肝脏组织总RNA为模板，通过RT-PCR扩增小鼠canstatin及其N端片段基因，克隆到pMD18-T载体中并进行序列分析。将小鼠canstatin及其N端片段基因定向克隆于原核表达载体pET30a(+)中,分别构建表达质粒pET/Can和pET/Can-N, 转化大肠杆菌BL21(DE3), IPTG诱导表达。结果表明: 小鼠canstatin的cDNA长度为684bp，编码227...
猪Ⅱ型圆环病毒ORF1基因克隆及在E.coli中的表达 。
Year-round Monitoring of Verotoxin-producing Escherichia coli from Feces of Dairy Cattle
Dairy Cattle Feces Verotoxin Genes Escherichia coli PCR Detection Diet Change
2016/4/25
A PCR-aided monitoring of verotoxin-producing Escherichia coli (VTEC) was performed over the period of 12 months by using fresh feces collected monthly from 5 dairy cows that had been identified as VT...
Effects of Egg Yolk Antibodies Produced in Response to Different Antigenic Fractions of E. coli O157:H7 on E. coli Suppression
E. coli O157:H7 Lipopolysaccharide Egg Yolk Antibody Chicken
2016/4/22
The objective of this research was to provide the characterization and method for producing anti-E. coli O157:H7 antibodies in egg-laying hens and to determine if the antibody can restrain the prolife...
Prevalence of Fimbrial Antigen (K88 variants, K99 and 987P) of Enterotoxigenic Escherichia Coli from Neonatal and Post-weaning Piglets with Diarrhea in Central China
Enterotoxigenic Escherichia Coil Fimbrial Antigen PCR Piglets Diarrhea
2016/4/21
Enterotoxigenic Escherichia coli is a major cause of diarrhea in neonatal and post-weaning piglets. To determine the most common fimbrial antigens of ETEC in piglets with diarrhea, two investigations ...
Improving Soluble Expression of β-Galactosidase in Escherichia coli by Fusion with Thioredoxin
Galactosidase Thioredoxin Inclusion Body IPTG
2016/4/18
Recombinant heterologous proteins can be produced as insoluble aggregates partially or perfectly inactive in Escherichia coli. One of the strateges to improve the solubility of recombinant proteins is...
Effects of Copper-bearing Montmorillonite (Cu-MMT) on Escherichia coli and Diarrhea on Weanling Pigs
Copper-bearing Montmorillonite Escherichia coil Diarrhea Intestinal Morphology Weanling Pigs
2016/4/18
Copper-bearing montmorillonite (Cu-MMT) was produced by Cu2+ cation exchange reaction. X-ray diffraction analysis showed that that the (001) basal spacing of the MMT crystal lattice increased from 1.5...
β-Galactosidase Gene of Thermus thermophilus KNOUC112 Isolated from Hot Springs of a Volcanic Area in New Zealand: Identification of the Bacteria, Cloning and Expression of the Gene in Escherichia coli
Thermus thermophilus Cloning and Expression
2016/4/18
To isolate the β-galactosidase producing thermophilic bacteria, samples of mud and water were collected from hot springs of avolcanic area near Golden Springs in New Zealand. Among eleven isolated str...
Production of Leptin in E. coli and Its Effect on Glucose and Acetate Transport and Expression of Uncoupling Protein-2 Gene in Adipose Tissues of Korean Cattle (Hanwoo)
Bovine Leptin 3T3-L1 Cells Adipose Tissues Glucose Transport Acetate Transport Uncoupling Protein-2
2016/4/15
Leptin has a major role in the regulation of food intake and energy homeostasis. In addition, leptin participates in many physiological functions including regulation of lipid metabolism. Bovine recom...