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Rapid Genotyping of MSTN Gene Polymorphism Using High-resolution Melting for Association Study in Rabbits
MSTN HRM Direct Sequencing Rabbits Production Traits
2016/5/11
The myostatin (MSTN) gene, as a negative regulator of skeletal muscle growth, has been proposed to be associated with production traits in farm animals. In the present study, a T/C variant at −1...
应用微卫星分型方法进行大熊猫父亲鉴定Paternity Assignment of Giant Panda by Microsatellite Genotyping
微卫星 基因分型 大熊猫 亲子鉴定
2007/12/31
摘要选用18对大熊猫特异的微卫星引物对成都动物园、成都大熊猫繁育研究基地的大熊猫幼子及其母亲和可疑父亲DNA作了PCR扩增。扩增产物在ABI 373测序仪上做基因扫描。 应用Genotyper 672 软件处理分型数据，经Cervus 2.0 软件对亲子关系进行分析。结果表明， 在母子关系确实的情况下，18个基因座联合的父亲鉴定排除概率E为0.999921，置信度为95%。而在母子关系不清的条件下...
Multilocus Genotyping to Study Population Structure in Three Buffalo Populations of India
Buffalo Microsatellites F-statistics Genetic Distance Correspondence Analysis
2016/4/21
Three buffalo populations viz. Bhadawari, Tarai and local buffaloes of Kerala were genotyped using 24 heterologous polymorphic microsatellite loci. A total of 140 alleles were observed with an average...
Three-step PCR and RFLP Genotyping of the Swine Ryanodine Receptor Gene Using Aged Single Hair Follicles Delivered by General Mail
Three-step (Booster and Nested) PCR Amplification RFLP Analysis Aged Hair Follicles RYR 1 Locus Genotyping
2016/4/11
We have developed a reliable and noninvasive method for swine genotyping of single locus nuclear gene with aged single hair follicles delivered by general mail. The method is based on booster and nest...