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In vitro Screening of Essential Oil Active Compounds for Manipulation of Rumen Fermentation and Methane Mitigation
Essential Oil Active Compounds Methane Production Volatile Fatty Acid Production
2016/7/27
The objective of this study was to investigate the effects of 11 active compounds of essential oils (ACEO) on rumen fermentation characteristics and methane production. Two trials were conducted. In t...
Reference Gene Screening for Analyzing Gene Expression Across Goat Tissue
Reference Gene Expression Stability Tissue Goat
2016/5/12
Real-time quantitative PCR (qRT-PCR) is one of the important methods for investigating the changes in mRNA expression levels in cells and tissues. Selection of the proper reference genes is very impor...
Isolation, Screening and Identification of Swine Gut Microbiota with Ochratoxin A Biodegradation Ability
Swine Intestinal Microbiota Enzyme-linked Immunosorbent Assay
2016/5/9
The potential for ochratoxin A (OTA) degradation by swine intestinal microbiota was assessed in the current study. Intestinal content that was collected aseptically from swine was spiked with 100 ppb ...
Identification and Screening of Gene(s) Related to Susceptibility to Enterotoxigenic Escherichia coli F4ab/ac in Piglets
ETEC F4ab/ac Susceptibility Receptor Muc4 Ep-CAM Piglets
2016/4/27
In 2004, J첩rgensen and coworkers proposed the MUC4 gene as a candidate gene of enterotoxigenic Escherichia coli (ETEC) F4ab/ac receptor in piglets and a mutation of G??C in intron 7 of MUC4 was...
Screening of Specific Genes Expressed in the Swine Tissues and Development of a Functional cDNA Chip
Swine Tissues cDNA Chip EST Microarray
2016/4/19
To develop a functional cDNA chip, specific genes expressed in the tissues of swine Kagoshima Berkshire were screened. A total of 4,434 ESTs were obtained by constructing a cDNA library from total RNA...
Rapid Screening of Salmonella spp. Using PBM BioSignTM Salmonella Test and Evaluation of the PBMS Test
PBM BioSignTM Salmonella (PBMS) Test Screening of Salmonella spp
2016/4/18
The PBM BioSignTM Salmonella (PBMS) test kit based on an immunochromatographic method was evaluated for the screening of Salmonella spp. in pure cultures, and 80, 15, and 10 artificially and naturally...
Screening and Characterization of Lactate Dehydrogenase-producing Microorganism
Lactobacillus Lactate Dehydrogenase Direct-fed Microbials
2016/4/18
The objective of this work was to isolate a microorganism, able to produce high lactate dehydrogenase (LDH) activity, for use as a microbial feed additive. The LDH is an important enzyme for lactate c...