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In Vivo Analysis of Human LHX3 Gene Regulation
Gene Regulation Pituitary Combined Pituitary Hormone Deficiency LHX3
2015/5/25
LHX3 is a transcription factor important in pituitary and nervous system development. Patients with mutations in coding regions of the gene have combined pituitary hormone deficiency (CPHD) that cause...
The LHX3 transcription factor is essential for pituitary gland and nervous system development in mammals. In humans, mutations in the LHX3 gene underlie combined pituitary hormone deficiency (CPHD) di...
In Vivo Labeling Of A Model β-Clam Protein With A Fluorescent Amino Acid
nonsense suppression technique L-(7-hydroxycoumarin-4-yl) ethyl glycine unnatural amino acid CRABP I
2014/12/12
Proteins can be labeled with different tags to enable their structural and functional investigations. In addition, labeling proteins at specific sites helps in studying the conformational dynamics of ...
人胰岛素基因在体内外表达的研究The Expression of Human Insulin Gene in vitro and in vivo
人胰岛素基因 基因治疗 肝细胞 糖尿病
2008/1/29
摘要利用定点突变人胰岛素基因，以脂质体载体和质粒不同比例形成的复合物进行体内外转染。体外转染鼠肝细胞，G418进行筛选，利用放免法测定培养液中胰岛素含量；体内通过肝门脉注射，测定模型鼠血糖及转染后7天时血液中胰岛素的含量，结果显示目的基因已转入肝细胞，且体内外转染都有一定量的成熟胰岛素表达，体外转染中质粒与脂质体比为1∶6转染后24h表达量最高为10.45μIU/ml，体内转染使模型鼠的糖尿病症状...