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Quantification of parvalbumin in commercially important Mediterranean seafood species using real time PCR
protein quantification Real Time PCR seafood allergy
2015/3/30
The parvalbumin allergen gene was quantified in various types of seafood using RT PCR. Freshly harvested specimens from 25 species of finfish, molluscs, and crustacean shellfish, commonly consumed in ...
Real-time PCR with internal amplification control for the detection of Cronobacter spp. (Enterobacter sakazakii) in food samples
Cronobacter spp. (Enterobacter sakazakii) Real-time PCR Internal amplification control Detection Food
2012/8/14
Cronobacter spp. (Enterobacter sakazakii) is an opportunistic pathogen and is linked with life-threatening infections in neonates. The organism has been isolated from a wide variety of foods and envir...
Changes in the Composition of the Bacterial Flora on Tray-Packaged Pork during Chilled Storage Analyzed by PCR-DGGE and Real-Time PCR
bacterial community Pseudomonas PCR-DGGE the CT method tray-packaged pork
2012/8/13
In this study, a polymerase chain reaction (PCR)-denaturing gradient gel electrophoresis (DGGE) was used to investigate the changes in the composition of the bacterial population of tray-packaged pork...
马铃薯Y 病毒是近年来危害烟草生产的重要病毒之一，严重影响烟草的产量与品质。本研究利用DNAMAN 软件对GenBank 数据库中已登录的马铃薯Y 病毒 (Potato virus Y, PVY) 全基因组序列进行序列比对，设计引物，以烟草肌动蛋白基因为内参，建立了烟草PVY 的实时定量检测体系。获得的real-time PCR 扩增基线平整，指数扩增明显，斜率大；稳定性和重现性好，变异系数小；循...
Quantitative Identification of Rice Cultivars by Real-Time PCR
rice Oryza sativa real-time PCR
2009/6/10
Application of a real-time PCR system for the detection of blending and the quantification of the blending ratio of rice was investigated. Quantitative measurement by real-time PCR was performed using...