搜索结果: 1-12 共查到“医学 Der f 1”相关记录12条 . 查询时间(0.015 秒)
粉尘螨变应原Der f 1 mRNA对小鼠特异性免疫治疗的实验研究
变应原 mRNA疫苗 特异性免疫治疗
2014/9/25
探讨粉尘螨变应原Der f 1 mRNA疫苗对哮喘小鼠的特异性免疫治疗效果。 方法 50只BALB/c小鼠随机分为5组(10只/组),分别为PBS组、Der f 1 变应原致敏组、Der f 1 变应原免疫治疗组、β-actin mRNA免疫治疗组和Der f 1 mRNA免疫治疗组。分别于第0、第7和第14天,PBS组小鼠腹腔注射PBS,其余4组小鼠则腹腔注射10 μg Der f 1进行致敏,...
粉尘螨变应原Der f Mag 29的克隆、表达及生物信息学分析
粉尘螨 变应原 生物信息学
2014/2/18
提取粉尘螨总RNA,根据GenBank中的序列(登录号为AY283281.1)设计并合成引物,RT-PCR扩增Mag 29全长基因,克隆至pCold TF DNA载体,转化至大肠埃希菌(E. coli)JM109,取阳性克隆测序鉴定。将pCold TF-Mag 29质粒转化至BL21,异丙基-β-D-硫代半乳糖苷(IPTG)诱导表达,十二烷基磺酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)验证表达产...
克隆和表达粉尘螨第7类变应原基因,并鉴定重组蛋白的免疫原性。 方法 提取粉尘螨总RNA,根据GenBank提供的Der f 7 编码区(CDS)(登录号为AY 283292)序列设计特异性引物,逆转录PCR(RT-PCR)克隆Der f 7基因。将测序正确的目的片段克隆至pET-32a表达载体,得到的重组质粒pET-32a-Der f 7在大肠埃希菌(E. coli)BL21(DE3)中用异丙...
粉尘螨变应原第1组分原核表达质粒pCold-TF-Der f 1构建及鉴定
尘螨变应原 基因重组 原核表达
2014/6/18
建立粉尘螨变应原第1组分全长基因原核表达质粒pCold-TF-Der f 1。方法 以质粒pET-28a(+)-Der f 1为模板扩增目的基因Der f 1,克隆至pCold-TF DNA载体,转化大肠埃希菌BL21,IPTG诱导表达并用SDS-PAGE(十二烷基磺酸钠-聚丙烯酰胺凝胶电泳)和蛋白印迹法(Western blotting,WB)验证产物。结果 PCR扩增获得Der f 1编码全长...
Associate Professor Marjolein C. H. van der Meulen,The Department of Biomedical Engineering at Cornell University(图)
Associate Professor Marjolein C. H. van der Meulen Biomedical Mechanics
2010/1/5
Associate Professor Marjolein C. H. van der Meulen,The Department of Biomedical Engineering at Cornell University.Her interest has refocused on the other role of mechanics: understanding how mechanica...
DIE HAEUFIGKELT DER MUNDSPEICHELDRUESENTUMOREN (MSDT) IM IRAN
HAEUFIGKELT MUNDSPEICHELDRUESENTUMOREN IRAN
2009/12/7
DIE HAEUFIGKELT DER MUNDSPEICHELDRUESENTUMOREN (MSDT) IM IRAN。
口咽胃肠道环境对Der p2-rBCG行为的影响
口咽胃肠道环境 Der p2-rBCG行为 影响
2009/7/30
目的: 观察口服接种过敏原基因(Der p2)重组(r)BCG口咽胃肠道环境对Der p2-rBCG行为的影响. 方法: 以野生BCG为对照,给予Balb/c小鼠口服接种三种方式(分泌、胞壁和胞内)表达Der p2的rBCG1X109CFU,连续5 d,计数不同时间粪便、口咽淋巴组织(OLT)、消化道相关淋巴组织(GALT)以及肝脏、脾脏和肺脏中BCG的细菌数;用PCR和内参照RT-PCR法测定由...
A Case of Partial Trisomy 13 Findings with 46,XX,der(7)t(7;13)(p22;q31)mat Karyotype
Trisomy 13 translocation partial trisomy
2009/6/30
Authors have shown that the trisomy of the distal part of chromosome 13 is related to different clinic findings than cases with classic trisomy 13 (1,2). They reported that different trisomic segments...
Genetic Impact of a Butyrophilin-like 2 (BTNL2) Gene Variation on Specific IgE Responsiveness to Dermatophagoides farinae (Der f) in Japanese
BTNL2 Dermatophagoides farinae (Der f) specific IgE
2010/11/16
Controlling for gender, age, smoking, and the presence of asthma, multiple logistic regression analyses showed that homozygosity of the rs2076530 A allele, which has been reported to be a risk allele ...
目的 构建粉尘螨6类变应原(Dermatophagoides farinae,Der f6)基因的高效原核表达载体,并进行表达、纯化及生物学功能分析。 方法 根据Der f6基因已知序列,设计1对引物,通过对纯培养的粉尘螨提取总RNA,采用RT-PCR方法扩增出Der f6基因片段,PCR产物克隆入pMD18-T载体,转化大肠埃希菌(E.coli Top10),经PCR和酶切鉴定并测序。将上述所得...
Message of PACE President René van der Linden on World AIDS Day(图)
World AIDS Day discrimination stigma
2006/12/1