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探究重组卡介苗(rBCGAg85AESAT6,rBCGAE)对人巨噬细胞免疫刺激活性的影响，为该疫苗的应用提供重要的理论依据和实验基础。方法将rBCGAE（rBCGAE感染组）及卡介苗（BCG感染组）分别感染体外培养的受PMA诱导分化的人单核细胞系（THP1细胞株），分别于感染后24、48及72 h，观察细胞表面分子CD80和CD86的表达及细胞培养基中干扰素（IFN）γ及肿瘤坏死...
Expression and Purification of Recombinant Mycobacterium Tuberculosis (TB) Antigens, ESAT-6, CFP-10 and ESAT- 6/CFP-10 and Their Diagnosis Potential for Detection of TB Patients
Mycobacterium tuberculosis ELISPOT CFP-10 ESAT-6 Tuberculosis Diagnosis
2015/9/23
Background: One of the most widely used methods to detect tuberculosis (TB) infection is the tuberculin skin test (TST). The completion of Mycobacterium tuberculosis (M. tuberculosis) genome sequence ...