搜索结果: 1-10 共查到“基础医学 RT”相关记录10条 . 查询时间(0.218 秒)
Screening of prognostic factors using multiplex RT-PCR technique on different leukemic cell lines
Multiplex RT-PCR T-ALL oncogene leukemia cell
2009/12/18
Background: Leukemia is one of the most common pediatric malignancies. T-cell Acute Lymphoblastic Leukemia (T-ALL) accounts for 15% of hematopoetic cancers. It has been well understood that identifica...
Comparison of Integrated Cell Culture -RT-PCR & Cell Culture Methods for Detection of Enteroviruses
Enteroviruses Sewage ICC-RT-PCR Iran
2009/11/26
Background: Generally, sewage exposed water could be potentially contaminated with enteroviruses. For this reason, enterovirus isolation from sewage specimens is one of the most sensitive indicat...
Quantification of The FLI1 Gene Expression By Real-Time Quantitative RT-PCR
Gene expression FLI1 PML-RARA Real Time RT-PCR
2009/6/25
In this study, quantification levels were investigated to define alterations in the expression of the FLI1 gene on acute promyelocytic leukemia (APL), which is characterized by a reciprocal t(15,17) t...
Investigating Toxic Effects of the HIV-RT Inhibitor 2-Phenoxymethyl-5-Chloro-Benzimidazole on Rat Liver
AIDS HIV-RT inhibitor hepatic toxicity histopathology 2-phenoxymethyl-5-chloro-benzimidazole dimethylsulfoxide
2009/6/23
The aim of this study was to investigate the toxic effects of the human immunodeficiency virus-reverse transcriptase (HIV-RT) inhibitor 2-phenoxymethyl-5-chlorobenzimidazole on rat liver at the light ...
联合应用实时定量RT-PCR和激光显微切割检测单个肝细胞RNA的表达（英文）
laser microdissction Real-time quantitative PCR RNA Hepatocytes
2009/5/8
联合应用实时定量RT-PCR和激光显微切割检测单个肝细胞RNA的表达（英文）。
RT-PCR检测亚马孙利什曼原虫P-4和GP-46基因表达
亚马孙利什曼原虫 无鞭毛体 前鞭毛体 阶段特异性蛋白
2009/2/26
目的 证明亚马孙利什曼原虫前鞭毛体和无鞭毛体的基因表达水平。 方法 用RNA分离试剂盒，分别提取3种不同来源的无鞭毛体（由小鼠模型皮损组织获得的无鞭毛体、由前鞭毛体培养转化而来的无鞭毛体, 以及来自J774.G8巨噬细胞株的无鞭毛体）的总RNA，以及前鞭毛体总RNA，然后用SuperScripⅡ逆转录聚合酶将其逆转录为cD-NA，再经PCR扩增无鞭毛体特异核酸酶（P-4）和前鞭毛体特异膜糖蛋白（G...
目的：比较烟曲霉毒力相关基因（pksP、fos-1、rhbA、paba、lysf、cpcA）在体内和体外表达上的差异，探讨毒力因子在感染宿主时的表达模式以及这种表达差异在烟曲霉致病方面的重要意义。材料与方法：1 菌株：本实验所用菌株为BMU1200号烟曲霉，为临床分离株。2 体外培养菌丝RNA的提取：调节孢子浓度为106/ml，接种于AMM液体培养基，37 ℃ 、220rpm培养24小时，收集菌丝...
高通量实时荧光定量RT-PCR方法验证FL细胞对苯并(a)芘7,8-二氢二醇9,10-环氧化物处理的差异表达基因
RT-PCR 低密度芯片 苯并(a)芘7,8-二氢二醇9,10-环氧化物 差异基因表达
2008/4/19
目的 验证由寡核苷酸微阵列检出的FL细胞对苯并(a)芘7,8-二氢二醇9,10-环氧化物(BPDE)处理的差异表达基因。方法 FL细胞分别经0.1％二甲亚砜（DMSO）和0.5 μmol·L-1 BPDE处理，以高通量实时荧光定量RT-PCR方法(TaqMan®低密度芯片)平行检测185个目标基因的相对表达水平。结果 BPDE处理组相对DMSO对照组，51个基因表达有差异，12个基因表...
IN VITRO SEMI-QUANTITATIVE DETERMINATAION OF HUMAN GAMMA-INTERFERON EXPRESSION BY RT-PCR
semi-quantitative IFN-γ PHA
2010/4/12
Secreted cytokines of Th1 (T-helper)/Th2 cells play an important role in the pathogenesis of many diseases. Th1 cells secrete predominantly IFN-γ and IL-2 which regulate cell-mediated immunity against...
RT-PCR检测肺癌患者外周血及区域淋巴结中CK19 mRNA的表达
肺癌 微转移
2013/12/16
应用RT-PCR法检测肺癌患者外周血和手术切除区域淋巴结中的微转移情况.方法 用逆转录PCR(reverse transcriptase polymerase chain reaction, RT-PCR)法检测78例肺癌患者和30例肺良性疾病患者外周血和手术切除淋巴结中CK19(cytokeratin 19) mRNA的表达,另抽取10例健康人外周血作为对照.对所有淋巴结进行常规病理切片HE染色...