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Significance of reactive oxygen species and antioxidants in defining the efficacy of sperm preparation techniques
reactive oxygen species antioxidants sperm preparation techniques
2009/2/23
The mechanisms responsible for mediating the influence of sperm preparation protocols on human sperm function have been investigated. Techniques that involved the separation of motile spermatozoa ...
Squash preparation studies of germ cells in human fetal testes
testis preleptotene germ cells chromosomes
2009/2/20
Testes from human fetuses 21 to 41 weeks of gestation were investigated using a modified squash technique. At all of the gestational ages examined, 90 to 95% of the germ cells were in interphase. Pr...
Preparation of Epithelial and Stromal Cell Fractions from Immature Rat Prostatic Tissue Using Percoll Gradients
prostate androgens testosterone
2009/2/19
A method is described for the dispersion, isolation, and partial characterization of epithelial and stromal tissue cells from the rat ventral prostate. Viable epithelial and stromal cells have bee...
Sperm function and choice of preparation media：comparison of Percoll and Accudenz discontinuous density gradients
Sperm maturity and creatine kinase motile sperm yield sperm activation chiortetracycline
2009/2/16
We compared the sperm populations prepared by Accudenz (35-65%) and Percoll (40-80%) density gradients in 21 normospermic specimens (concentration, 53.6 +/- 3.8 x 10(6) sperm/ml; motility, 44.5 +/...
Preparation and characterization of monoclonal antibodies against sperm-specific lactate dehydrogenase C4
LDH-C4 monoclonal antibodies specificity
2009/2/6
LDH-C4, the testis-specific isozymes of lactate dehydrogenase (LDH), is the predominant LDH isozyme in mammalian spermatozoa. Nine monoclonal antibodies against mouse LDH-C4 have been developed. The...
Preparation and short-term culture of enriched populations of guinea pig spermatocytes and spermatids
spermatogenesis spermatocyte spermatid
2009/2/6
In order to study acrosome biogenesis in vitro, the authors developed techniques for the isolation and culture of enriched populations of guinea pig spermatogenic cells using a modification of the t...
Sperm Preparation Methods
Sperm Preparation Methods
2008/12/25
The spermatozoa of all placental (eutherian) mammals,
including humans, are in a protective, nonlabile state at
ejaculation and are incapable of fertilization even if they
are placed in direct cont...
Recipient Preparation and Mixed Germ Cell Isolation for Spermatogonial Stem Cell Transplantation in Domestic Cats
Testis male radiation feline conservation
2008/12/23
The loss of genetic diversity poses a serious threat to the conservation of endangered species, including wild felids. We are attempting to develop spermatogonial stem cell transplantation in the cat ...
Depletion of Endogenous Germ Cells in Male Pigs and Goats in Preparation for Germ Cell Transplantation
Irradiation busulfan testis transgenesis large animals
2008/12/23
The efficiency of germ cell transplantation, the procedure of transferring germ cells from a donor male into the testes of recipient males, can be greatly increased by reduction of endogenous germ c...
Practical Considerations in the Preparation of Sperm Prior to Cryopreservation
Practical Considerations Preparation Sperm Cryopreservation
2008/12/23
Clinicians continue to develop a broader understanding of the utility of cryopreserving sperm in a variety of settings. Such settings range from the preservation of sperm before initiation of chemothe...
Sperm Morphology and Preparation Method Affect Bovine Embryonic Development
Percoll swim-up abnormal spermatozoa thermal insult in vitro fertilization
2008/12/22
This study was conducted to evaluate the effect of sperm separation methods of semen samples collected from bulls subjected to scrotal insulation on embryonic development after in vitro fertilizat...