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Validation of a Direct Radioimmunoassay for Testosterone in Unextracted Serum from Five Species: Application to Study of the Hypothalamic-Pituitary-Gonadal Axis in Males
direct radioimmunoassay serum testosterone luteinizing hormone releasing hormone immunoneutralization
2009/2/19
A method for the radioimmunoassay of serum testosterone that does not require extraction or chromatography is described. Utilization of a highly specific antiserum, tritiumlabeled ligand, and doub...
Spermatogenesis in Men Treated with Subcutaneous Application of Levonorgestrel and Estrone Rods
spermatogenic arrest silastic rods hormone-impregnated subcutaneous implantation
2009/2/19
The effects of a combination of a progestin, levonorgestrel (LNg), and estrone (E1), on exocrine and endocrine testicular function were examined in adult men. The mode of administration was subc...
A new method to generate canine seminal emission and its application to men：direct electrical stimulation of the vas deferens
generate canine seminal emission direct electrical stimulation vas deferens
2009/2/12
Seminal emission from the ejaculatory duct (SEED) by direct electrical stimulation of the vas deferens was investigated in the dog, and the technique was applied to men. The stimulus parameters used w...
The application of pentoxifylline in the stimulation of sperm motion in men undergoing electroejaculation
Spinal cord injury electroejaculation sperm
2009/2/7
The ability of pentoxifylline to stimulate the motion characteristics of antegrade and retrograde sperm collected at the time of electroejaculation with a rectal probe was assessed in six neurologic...
Application of flow cytometry to studies on the human acrosome
flow cytometry lectin acrosome normooospermia acrosome reaction
2009/2/6
This study describes the use of flow cytometry combined with specific labelling of the human sperm acrosome using a FITC-labelled plant lectin (Arachis hypogea agglutinin). Localization of the label...
Measurement of Volume Changes in Mouse Spermatozoa Using an Electronic Sizing Analyzer and a Flow Cytometer: Validation and Application to an Infertile Mouse Model
Coulter counter c-ros knockout mice hypo-osmotic swelling regulatory volume decrease
2008/12/5
The importance of sperm volume has recently been highlighted in a knockout mouse model in which infertility was caused by defects in volume reguiation, which led to sperm transport failure in the fe...
The Application of Computer Content Analysis in Sexology: A Case Study of Primary Process Content in Fictional Fetishistic Narratives
Fictional Fetishistic Narratives Primary Process Content
2008/5/6
This paper illustrates an application of computer content analysis in sexology. It compares a set of fictional fetishistic narratives published on a web site for rubber boot fetishists (n = 27) with a...