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A Method for the Determination of Chlorinated Pesticides in Human Semen
chlorinated pesticide human semen gas chromatography
2009/2/19
A method is described for the quantitative and qualitative determination of BHC and DDT isomers and HCB and Methoxychlor (DMDT) in human semen. Pesticide residues were extracted with petroleum eth...
Determination of the steady-state intracellular chloride concentration in capacitated human spermatozoa
Methoxy-N-ethylquinolonium N-(6-methoxyquinolyl)
2009/2/18
Chloride channels participate in the mammalian sperm acrosome reaction (AR). However, the mechanism by which sperm regulate intracellular Cl- concentration ([Cl-]i) is not known. The steady-state ...
The fix vital stain method. Simultaneous determination of viability and acrosomal status of bovine spermatozoa
Acrosomal evaluation vital staining Hoechst bisbenzimide
2009/2/7
A rapid, accurate and precise method for simultaneous determination of acrosomal status and viability of bull spermatozoa is introduced and evaluated. The method involves fixation of semen with glut...
Standardization and Quality Control for Determination of Fructose in Seminal Plasma
Standardization Quality Control Fructose Seminal Plasma
2008/12/24
In this age of evidence-based medicine, there is nothing more important than the quality of lab tests (Westgard and Darcy, 2004). However, reliability of semen analysis has been suspected for lack o...
Could Sperm Aneuploidy Rate Determination Be Used as a Predictive Test Before Intracytoplasmic Sperm Injection?
Chromosomal profile fluorescent in situ hybridization spermatozoa
2008/12/23
Chromosome abnormalities in embryos are a major cause of implantation and development failures. Some couples with normal karyotypes have repeated implantation failures after intracytoplasmic sperm...
A Flow Cytometric Method for Rapid Determination of Sperm Concentration and Viability in Mammalian and Avian Semen
Cytometry spermatozoa count vitality precision accuracy
2008/12/22
A new flow cytometric method has been developed to rapidly determine sperm concentration and viability in semen from bulls and boars. Sperm viability was determined on the basis of staining with SYB...
The Sperm Chromatin Dispersion Test: A Simple Method for the Determination of Sperm DNA Fragmentation
Structure human sperm decondensation
2008/12/18
Sperm DNA fragmentation is being increasingly recognized as an important cause of infertility. We herein describe the Sperm Chromatin Dispersion (SCD) test, a novel assay for sperm DNA fragmentation...