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Secretion of plasminogen activator in response to follicle-stimulating hormone in culture medium of human testicular cells from biopsy specimens
plasminogen activator follicle-stimulating hormone culture medium human testicular cells
2009/2/23
Testicular cells from human biopsy specimens were cultured and plasminogen activator secreted into the medium was investigated. Follicle stimulating hormone stimulated plasminogen activator secret...
Inhibition of ornithine decarboxylase activity by follicle stimulating hormone in primary culture of rat Sertoli cells
ornithine decarboxylase follicle stimulating
2009/2/23
The effect of follicle stimulating hormone on the activity of ornithine decarboxylase (ODC) was determined in primary culture of rat Sertoli cells. Three different FSH preparations (NIH oFSH-S-15, S...
Transferrin-mediated transcellular transport of 59Fe across confluent epithelial sheets of Sertoli cells grown in bicameral cell culture chambers
Transferrin-mediated transcellular transport 59Fe Sertoli cells bicameral cell
2009/2/23
The transferrin-mediated transcellular transport of 59Fe across confluent epithelial sheets of Sertoli cells grown on Millipore filters was investigated. These filters had been impregnated with reco...
Polarized Sertoli cell functions in a new two-compartment culture system
Polarized Sertoli cell functions new two-compartment culture system
2009/2/23
Sertoli cells in vivo are highly polarized and interact with the inner (tubular) and outer (interstitial) fluids. To simulate this condition in vitro we developed a two-compartment culture system ...
Growth and characterization of polarized monolayers of epididymal epithelial cells and Sertoli cells in dual environment culture chambers
polarized monolayers epididymal epithelial cells Sertoli cells dual environment culture chambers
2009/2/23
Epididymal epithelial cells isolated from mature rats and Sertoli cells isolated from 10-day-old rats were cultured in serum-free defined media on extracellular matrix impregnated filters maintained...
Culture of ciliated and nonciliated cells from rat ductuli efferentes
ciliated and nonciliated cells rat ductuli efferentes
2009/2/23
The isolation and culture of ciliated and nonciliated cells from rat ductuli efferentes is described. Fragments of epithelium obtained after two collagenase digestions attached to plastic and to ext...
Culture of principal cells from the ram epididymis
principal cells ram epididymis morphology
2009/2/20
A procedure was developed to isolate and culture principal cells from the initial segment, central caput, distal caput, and proximal corpus epididymidis. The morphology of cultured cells and of cell...
Organ culture of rat caput epididymal tubules in a perifusion chamber
Organ culture rat caput epididymal tubules perifusion chamber
2009/2/20
We have been able to culture caput epididymal tubules from rats by modifying an organ culture system (Orgebin-Crist et al, 1980) which was used to culture rabbit corpus epididymal tubule segments. T...
Effects of gossypol on rat Sertoli and Leydig cells in primary culture and established cell lines
rat Sertoli Leydig cells established cell lines
2009/2/19
In order to evaluate the direct effect of gossypol on testicular cells, we used primary cultures of rat Leydig and Sertoli cells. No alteration in Leydig cell survival, morphology, or testosterone...
Catecholamine Stimulation of Androgen Production by Mouse Interstitial Cells in Primary Culture
catecholamines Leydig cells androgens cell culture
2009/2/19
The catecholamines isoproterenol, epinephrine, and norepinephrine stimulated androgen production by mouse interstitial cells in primary culture. The amount of androgen produced in response to maximu...
A Continuous Flow Method for Organ Culture of Rabbit Epididymis：Morphology, Amino Acid Utilization, Glucose Uptake, RNA, and Protein Synthesis
epididymis organ culture glucose uptake amino acid utilization
2009/2/18
A modified perfusion system was developed to maintain an unconvoluted tubule from the corpus epididymidis of the rabbit in organ culture. The epididymal explant is submerged in a continuous flow of ...
The objective of this study was to identify the cells from rat prostate epithelium that attach and proliferate in primary culture. Minced ventral prostate was dissociated by DNAse/collagenase dige...
Catalase and oviductal fluid reverse the decreased motility of bovine sperm in culture medium containing specific amino acids
Hydrogen peroxide oviduct secretions toxicity
2009/2/17
The motility and velocity of bovine spermatozoa incubated in TCM-199 are reduced in comparison with those incubated in a simpler media made for sperm, such as modified Tyrode (Sp-Talp). Moreover, ...
Sertoli cells in culture and mRNA differential display provide a sensitive early warning assay system to detect changes induced by xenobiotics
Sertoli cells culture mRNA differential display
2009/2/17
We have used cultured rat Sertoli cells as an "early warning system" to monitor for morphological and biochemical changes induced by two different xenobiotics-cadmium acetate and polychlorinated bip...
Effect of TGF-beta 1, TGF-alpha, and EGF on cell proliferation and cell death in rat ventral prostatic epithelial cells in culture
Prostate epithelium TGF-91 TGF-a EGF apoptosis
2009/2/13
A tissue culture system for rat prostatic epithelial cells was developed, and the effect of epidermal growth factor (EGF), transforming growth factor alpha (TGF-alpha), and transforming growth facto...