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分析不同血清类型的类风湿性关节炎（RA）患者血清中的类风湿性关节炎特异性抗原瓜氨酸化蛋白（RACP） 的水平，比较RA-CP 与抗环瓜氨酸多肽（CCP）抗体、类风湿因子（RF）在RA 诊断中的价值。方法 用酶联免疫 吸附法（ELISA）检测103 例RA 患者、71 例其他风湿病患者、105 例健康体检者血清中RA-CP，抗CCP 抗体和RF 的 水平。根据抗CCP 抗体和RF 测定结果将RA 患...
为明确辣椒叶脉黄化病毒（Pepper vein yellows virus，PeVYV）在贵州辣椒上的发生分布及其分子变异情况。用RT-PCR法对采自贵州省25个采样地点的548份辣椒疑似病毒病样品进行检测。结果表明：129份为阳性样品，检出率为23.54%。将获得的14个不同采样点PeVYV分离物和已报道的中国和其他国家PeVYV分离物进行序列比对，P0基因核苷酸同源性为90.9% ~ 100....
机器人的CP-nets优化类人轨迹规划
机器人控制 人机系统 条件偏好网 轨迹
2019/3/15
机器人移动轨迹按照人的手臂来模拟是提高机器人安全性和人机交互能力的有效方法，特别是针对机器人抓取路径不唯一的场合，类人行为对于人机系统表现更加自然。此前，通常利用Kinect等设备，基于人工神经网络和K近邻算法等智能算法对类人轨迹进行规划，但无法获得未采样过的最优轨迹。本文基于CP-nets采用偏好模型研究类人运动轨迹，然后将该模型应用于机器人控制，在没有采样的情况下，也可得到最优的类人轨迹。实验...
小麦FtsH2蛋白与WYMV CP互作研究
FtsH2 小麦黄花叶病毒 CP AAA蛋白酶
2019/12/25
小麦黄花叶病毒(Wheat yellow mosaic virus, WYMV)是马铃薯Y病毒科大麦黄花叶病毒属成员,主要危害冬小麦。实验室前期以WYMV外壳蛋白(coat protein,CP)为诱饵,通过酵母双杂交筛选小麦cDNA文库,发现FtsH2蛋白部分片段与WYMV CP互作。FtsH2蛋白是AAA蛋白酶家族成员,参与植物叶绿体光损伤修复和类囊体发育进程。本研究利用酵母双杂交和双分子荧光...
IR-CP-ABE: Identity Revocable Ciphertext-Policy Attribute-Based Encryption for Flexible Secure Group-Based Communication
Group-based Secure Communication Ciphertext-Policy Attribute-Based Encryption ID Revocation
2017/11/20
Ciphertext-Policy Attribute-Based Encryp- tion (CP-ABE) is an access control mechanism over encrypted data and well suited for secure group-based communication. However, it also suffers from the fol- ...
HIR-CP-ABE: Hierarchical Identity Revocable Ciphertext-Policy Attribute-Based Encryption for Secure and Flexible Data Sharing
Attribute-Based Access Control Encryption CP-ABE
2017/11/20
Ciphertext Policy Attribute-Based Encryption (CP- ABE) has been proposed to implement the attribute-based access control model. In CP-ABE, data owners encrypt the data with a certain access policy suc...
CP-consensus: a Blockchain Protocol Based on Synchronous Timestamps of Compass Satellite
blockchainBlockchain consensus throughput
2017/11/1
Bitcoin, the first decentralized cryptocurrency, achieves great success but also encounters many challenges. In this paper, we mainly focus on Bitcoin's five challenges: low network synchronization; p...
以感病紫茉莉叶片为材料，采用RT-PCR方法克隆落葵皱缩花叶病毒（Basella rugose mosaic virus，BaRMV）的外壳蛋白（coat protein，CP）基因，并构建pET30a-BaRMV-cp原核表达载体。将原核表达载体转化大肠杆菌[Rosetta(DE3)PlysS]，成功诱导表达得到大小约为40.0 kD的与预期大小相符的融合蛋白（His-CP）。SDS-PAGE电...
对云南嵩明百合上发生的百合斑驳病毒(Lily mottle virus, LMoV)进行全基因组序列测定及分析,并对LMoV嵩明分离物(LMoV-SMi1、LMoV-SMi2)和玉溪分离物(LMoV-YXi1、LMoV-YXi2)外壳蛋白(coat protein,CP)基因进行序列比较,发现云南的LMoV分为2个类群,玉溪分离物属于种群I,嵩明分离物属于种群II。2个类群间的核苷酸和氨基酸同源性...
黄瓜花叶病毒(CMV)2个山西分离物CP序列测定及亚组分类分析
番茄 黄瓜花叶病毒 序列分析 亚组分类分析
2020/2/18
在生物学检测的基础上,利用酶联免疫检测、非序列依赖性PCR(sequence-independent amp-lification, SIA)对感病番茄进行分子鉴定,表现卷叶和花叶症状的番茄均被黄瓜花叶病毒(Cucumber mosaic virus,CMV)侵染,分离物分别命名为SXFQ(GenBank登录号为JX993914)和FQ(GenBank登录号为JX993912)。为明确其分类地位,...
An Efficient CP-ABE with Constant Size Secret Keys using ECC for Lightweight Devices
Attribute-based encryption ciphertext-policy constant-size secret key
2015/12/23
The energy cost of asymmetric cryptography is a vital component of modern secure communications, which
inhibits its wide spread adoption within the ultra-low energy regimes such as Implantable Medica...
Traceable CP-ABE on Prime Order Groups: Fully Secure and Fully Collusion-resistant Blackbox Traceable
Traceable Ciphertext-policy Attribute Based Encryption Prime Order Groups
2015/12/23
In Ciphertext-Policy Attribute-Based Encryption (CP-ABE), access policies associated with
the ciphertexts are generally role-based and the attributes satisfying the policies are generally shared by
...
采用两种策略增强了马铃薯卷叶病毒（PLRV）CP 基因的水溶性原核表达。首先，用5 种可表达出分子伴侣的质粒分别转化重组菌TOP10（pBAD-LRCP），获得了既含pBAD-LRCP 又含分子伴侣质粒的转化子；诱导转化子中PLRV-CP基因与分子伴侣基因同时表达，SDS-PAGE 结果表明，从转化子提取的水溶性蛋白中有明显的PLRV 重组CP 条带，即分子伴侣增进了重组CP 的水溶性表达。其次，...
Bioassay and Phylogeny of Five Iranian Isolates of Cucumber mosaic virus from Different Hosts Based on CP Gene Sequence
CMV coat protein genetic variation phylogeny symptomology subgroup I
2015/10/9
Using double-antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA), Cucumber mosaic virus (CMV)
was detected in 31 out of 132 symptomatic leaf samples collected from different hosts of Urm...