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Rapid Identification of Bifidobacteria in Dairy Products by Gene-targeted Species-specific PCR Technique and DGGE
Bifidobacterium spp. Species-specific PCR DGGE
2016/4/27
This study was conducted to examine the effects of different soy-oil and soy-lecithin levels on growth performance, nutrient utilization and serum parameters in broiler chickens. Two hundred and forty...
Rapid Identification of Transgenic Cotton (Gossypium hirsutum L.) Plants by Loop-mediated Isothermal Amplification
Bt chitinase CryIA(b) detection loop-mediated isothermal amplification transgenic cotton
2014/2/25
In an attempt to speed up the process of screening of transgenic cotton (G. hirsutum L.) plants, a visual and rapid loop-mediated isothermal amplification (LAMP) assay was adopted. Genomic DNA was ext...
苦瓜MAP30基因原核表达载体的构建和PCR快速检测重组子的研究 Construction of Prokaryotic Expression Vector for MAP30 Gene and Study of PCR Methods for Rapid Identification of Recombinant
MAP30 PCR 克隆子筛选 苦瓜
2008/1/8
摘要
根据已报道的序列，把苦瓜(Momordica charantia) MAP30基因成功克隆到原核表达载体pET28a（+）中，并对PCR快速检测阳性克隆进行了研究。结果表明，直接用菌落、菌液、酚氯仿处理过的菌液,以及提取的质粒进行PCR都可以成功地筛选阳性菌落。其中，酚氯仿处理过的菌液PCR与质粒PCR的结果最接近，而且比质粒PCR简单，因此可作为方便可靠的阳性克隆筛选的新方法。Abstr...